Eisenbarth G S, Shimizu K, Bowring M A, Wells S
Proc Natl Acad Sci U S A. 1982 Aug;79(16):5066-70. doi: 10.1073/pnas.79.16.5066.
Studies of the reaction of antibody A2B5 and tetanus toxin with pancreatic islet cells, islet cell tumors, and other human amine precursor uptake and decarboxylation (APUD) tumors are described. By indirect immunofluorescence, antibody A2B5 and tetanus toxin were shown to specifically bind to the plasma membrane of human, rat, chicken, and mouse islet cells. The binding of antibody A2B5 to the cell surface of living islet cells has allowed isolation of these cells from a suspension of pancreatic cells by using a fluorescence-activated cell sorter. In studies designed to determine whether tetanus toxin and antibody A2B5 bound to the same surface antigen, A2B5 and tetanus toxin did not compete for binding to normal islet cells, a human islet cell tumor, or a rat islet cell tumor. In addition to binding to islet cell tumors, antibody A2B5 reacts with frozen sections, isolated cells, and cell lines of neural, neural crest, and APUD origin.
本文描述了抗体A2B5和破伤风毒素与胰岛细胞、胰岛细胞瘤以及其他人类胺前体摄取和脱羧（APUD）肿瘤的反应研究。通过间接免疫荧光法，发现抗体A2B5和破伤风毒素能特异性结合人、大鼠、鸡和小鼠胰岛细胞的质膜。抗体A2B5与活胰岛细胞质膜的结合使得可以利用荧光激活细胞分选仪从胰腺细胞悬液中分离出这些细胞。在旨在确定破伤风毒素和抗体A2B5是否结合同一表面抗原的研究中，A2B5和破伤风毒素在与正常胰岛细胞、人胰岛细胞瘤或大鼠胰岛细胞瘤的结合上不存在竞争。除了与胰岛细胞瘤结合外，抗体A2B5还能与神经、神经嵴和APUD来源的冰冻切片、分离细胞及细胞系发生反应。
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