Brogren C H, Hirsch F, Wood P, Druet P, Poussier P
Diabetologia. 1986 May;29(5):330-3. doi: 10.1007/BF00452071.
Islet cell surface autoantibodies are present in the serum of the spontaneously diabetic BB rat. The availability in large quantities of such autoantibodies should help us understand their significance in vivo. Fusions between BB rat lymphocytes and rat myeloma cells were screened by cellular enzyme linked immunosorbent assay and indirect immunofluorescence on rat living cells. They resulted in a stable hybridoma, called IC2, secreting a monoclonal immunoglobulin M specific for the surface of rat islet cells. This monoclonal antibody was found to bind to the surface of 56% normal rat islet cells and 72% rat insulinoma cells. Protease treatment of rat islet cells resulted in a subsequent 72-100% binding inhibition of IC2 to the surface of these cells, suggesting that IC2 specific antigen is a protein.
胰岛细胞表面自身抗体存在于自发性糖尿病BB大鼠的血清中。大量获得此类自身抗体将有助于我们了解它们在体内的重要性。通过细胞酶联免疫吸附测定法以及对大鼠活细胞进行间接免疫荧光，对BB大鼠淋巴细胞与大鼠骨髓瘤细胞之间的融合体进行筛选。结果得到了一种稳定的杂交瘤，称为IC2，它分泌一种针对大鼠胰岛细胞表面的单克隆免疫球蛋白M。发现这种单克隆抗体可与56%的正常大鼠胰岛细胞表面以及72%的大鼠胰岛素瘤细胞表面结合。用蛋白酶处理大鼠胰岛细胞后，IC2对这些细胞表面的结合抑制率随后达到72% - 100%，这表明IC2的特异性抗原是一种蛋白质。
