Rosenkranz B, Hofbauer K, Fischer C, Frölich J C
Ren Physiol. 1982;5(4):186-91. doi: 10.1159/000172856.
Rabbit kidneys were isolated and perfused with a solution to which tritiated or unlabeled prostacyclin was added continuously. The ureteral and venous effluents were collected separately and chromatographed. The peaks obtained by high-pressure liquid chromatography were analyzed using gas chromatography-mass spectrometry. The characteristic fragments of dinor-6-keto-PGF1 alpha could be detected from ureteral and venous effluents and represented material corresponding to 9.6 and 9.1% of the radioactivity recovered from chromatography, respectively. In addition, 39.2% (ureteral effluent) and 58.2% (renal venous effluent) of the radioactivity could be identified as 6-keto-PGF1 alpha, the stable in vitro hydrolysis product of prostacyclin. These results show that the kidney is able to degrade prostacyclin by beta-oxidation and that dinor-6-keto-PGF1 alpha formed in the kidney can be detected in both the vascular and urinary compartments.
分离出兔肾，并用持续添加了氚标记或未标记前列环素的溶液进行灌注。分别收集输尿管和静脉流出液，并进行色谱分析。使用气相色谱 - 质谱联用仪对高压液相色谱获得的峰进行分析。从输尿管和静脉流出液中可检测到二羟 - 6 - 酮 - PGF1α 的特征性片段，分别占色谱回收放射性物质的9.6%和9.1%。此外，39.2%（输尿管流出液）和58.2%（肾静脉流出液）的放射性物质可鉴定为6 - 酮 - PGF1α，即前列环素的体外稳定水解产物。这些结果表明，肾脏能够通过β氧化降解前列环素，并且在肾脏中形成的二羟 - 6 - 酮 - PGF1α 可在血管和尿液成分中检测到。
