Floyd D M, Huang S T, Poon M C
Transfusion. 1982 Sep-Oct;22(5):352-4. doi: 10.1046/j.1537-2995.1982.22583017455.x.
The peroxidase-anti-peroxidase immunoenzyme method was applied to red blood cell ghosts for the detection of ABO mixed-red blood cell populations. Red blood cell ghosts produced by acid-glycine lysis eliminated previous problems due to distorted red blood cell and hemoglobin-associated peroxidase activity. Anti-A,B was used to discriminate between various mixtures of test cells in group O cells. The positive-stained minor population of test cells stained dark brown following application of the peroxidase-anti-peroxidase method and the nonreactive group O cells were counterstained light blue with Coomassie brilliant blue which facilitated quantitation. The expected and observed numbers of positive-stained cells per 1000 cells were not significantly different as computed by X2. As low as a 0.5 percent minor population in an ABO red blood cell mixture could be accurately quantitated by this method.
采用过氧化物酶-抗过氧化物酶免疫酶法检测红细胞空壳中的ABO混合红细胞群体。酸-甘氨酸裂解产生的红细胞空壳消除了因红细胞变形和血红蛋白相关过氧化物酶活性而产生的先前问题。使用抗A、B来区分O型细胞中测试细胞的各种混合物。应用过氧化物酶-抗过氧化物酶法后，测试细胞中呈阳性染色的少数群体染成深棕色，未反应的O型细胞用考马斯亮蓝复染成浅蓝色，便于定量。通过X2计算，每1000个细胞中预期和观察到的阳性染色细胞数量无显著差异。用这种方法可以准确地定量ABO红细胞混合物中低至0.5%的少数群体。
