Binding of a bacteriophage to wall-membrane adhesion in proteus mirabilis.

A bacteriophage was shown to adsorb to plasmolyzed non-swarming cells of Proteus mirabilis preferentially at the sites of adhesion between the inner membrane and outer cell wall membrane; 75% of phage particles were adsorbed at these sites, while 25% were not. Differences in outer membrane composition between swarming and non-swarming cells were reflected in altered phage-binding properties, with only 33% of phage absorbed at these adhesion sites in swarming cells. On the basis of their phage distribution, cross-sections of swarm cells could be distinguished from sections of short non-swarming cells.