Armitage J P
J Bacteriol. 1982 May;150(2):900-4. doi: 10.1128/jb.150.2.900-904.1982.
Freeze-fracture studies of short, nonswarming Proteus mirabilis revealed the characteristic gram-negative profile of fractured inner membrane with densely packed particles and sectioned outer membrane with little or no fracture plane. Long swarming cells, however, fractured easily along both the inner membrane and a second membrane, probably the outer membrane. The inner membrane had a typical profile, whereas the outer membrane had fewer but more prominent particles. Isolation and purification of the inner and outer membranes of the short and long bacteria and examination of them with electron paramagnetic resonance measurements after spinlabeling supported the above observations. The outer membrane of swarmer cells allowed higher mobility of the spin label than did the outer membrane of the nonswarming short cells, which showed a typical rigid profile. These results suggest that regions of lipid bilayer appear in the outer membrane during swarmer formation. Previous observation of the behavior and biochemistry of P. mirabilis during swarming are discussed in light of these results.
对短时间内不群集的奇异变形杆菌进行的冷冻断裂研究显示，断裂的内膜呈现出典型的革兰氏阴性菌特征，有紧密堆积的颗粒，而切片的外膜几乎没有或没有断裂平面。然而，长的群集细胞很容易沿着内膜和第二层膜（可能是外膜）断裂。内膜具有典型的轮廓，而外膜的颗粒较少但更突出。对短时间和长时间细菌的内膜和外膜进行分离和纯化，并在自旋标记后用电子顺磁共振测量对其进行检查，支持了上述观察结果。群集细胞的外膜比不群集的短细胞的外膜允许自旋标记有更高的流动性，后者呈现出典型的刚性轮廓。这些结果表明，在群集细胞形成过程中，外膜出现了脂质双层区域。根据这些结果，讨论了之前对奇异变形杆菌群集行为和生物化学的观察。
