Röhnert C, Weber K, Schuster R, Lauschke G, Häcker R
Z Exp Chir. 1982 Jul;15(4):202-9.
The metabolic state of the liver during preservation as well as in and after auxiliary transplantation was investigated in dogs. The biopsy was carried out by means of TRU-CUT biopsy needle. - The ATP concentration, the storage of energy, the amount of the adenine nucleotides, and the ATP/ADP quotient decrease in the preserved liver. The AMP concentration, the concentration of the hexosemonophosphates and the mass-effective quotient of the phosphofructokinase increase simultaneously. The energy supply is the glycolysis exclusively. A lactate accumulation is avoided by continuous perfusion. - The changeover of the anaerobic to the aerobic energy preparation took place by degrees after the recovery of the liver circulation. The ATP concentration, the ATP/ADP quotient, the storage of energy, and the amount of the adenine nucleotides increase in the transplanted liver. - An irreversible ischemic damage of the liver appears in the metabolic state of the organ by a massive decrease of the ATP concentration below 0.7 mmol/kg, the amount of the adenine nucleotides below 1.8 mmol/kg, and AMP concentration.
对犬类肝脏在保存期间以及辅助移植过程中和移植后的代谢状态进行了研究。活检通过TRU-CUT活检针进行。——在保存的肝脏中，ATP浓度、能量储备、腺嘌呤核苷酸量以及ATP/ADP比值均降低。AMP浓度、己糖单磷酸浓度以及磷酸果糖激酶的质量效应比值同时升高。能量供应仅靠糖酵解。通过持续灌注避免乳酸积累。——肝脏循环恢复后，无氧能量准备逐渐转变为有氧能量准备。移植肝脏中的ATP浓度、ATP/ADP比值、能量储备以及腺嘌呤核苷酸量均增加。——当ATP浓度大幅降至0.7 mmol/kg以下、腺嘌呤核苷酸量降至1.8 mmol/kg以下以及AMP浓度出现时，肝脏会出现不可逆的缺血损伤。
