Muccino R R, Vernice G G, Cupano J, Oliveto E P, Liebman A A
Steroids. 1978 May;31(5):645-52. doi: 10.1016/s0039-128x(78)80004-x.
Catalytic tritium reduction of cholest-5-en-23-yne-3beta,25-diol diacetate (VIII) gave cholest-5-ene-3 beta,25-diol diacetate-23,23,24,24-t4 (IX) having a specific activity of 92 Ci/mmol. Bromination, dehydrobromination and hydrolysis of the labelled material gave cholesta-5,7-diene-3beta,25-diol-23,23,24,24-t4 (XI) which was photolyzed to the previtamin and then thermally equilibrated to 25-hydroxycholecalciferol-23,23,24,24-t4 (I).
胆甾-5-烯-23-炔-3β,25-二醇二乙酸酯（VIII）的催化氚还原反应生成了比活度为92 Ci/mmol的胆甾-5-烯-3β,25-二醇二乙酸酯-23,23,24,24-t4（IX）。标记物的溴化、脱溴化氢和水解反应生成了胆甾-5,7-二烯-3β,25-二醇-23,23,24,24-t4（XI），其经光解生成前维生素，然后热平衡为25-羟基胆钙化醇-23,23,24,24-t4（I）。
