一种用于测定鱼血浆中11-氧代睾酮的放射气相色谱法：其在通过放射免疫测定法进行确认估计中的应用。

A radio-gas chromatographic method for the determination of 11-oxotestosterone in fish plasma: its use in confirming estimations by radioimmunoassay.

作者信息

Simpson T H, Wright R S

出版信息

Steroids. 1978 May;31(5):691-5. doi: 10.1016/s0039-128x(78)80009-9.

DOI:10.1016/s0039-128x(78)80009-9

Abstract

A radio-gas chromatographic method has been devised for the estimation of 11-oxotestosterone (17beta-hydroxyandrost-4-ene-3, 11-dione) in fish plasma samples which provides an independent means of validating the radioimmunoassay described earlier. Estimates of the concentration of 11-oxotestosterone in a sample of male rainbow trout plasma by radio-gas chromatography using peak height and peak weight measurements were 6.9 microgram/100 ml and 7.4 microgram/100 ml respectively, in good agreement with that of 7.1 microgram/100 ml determined by radioimmunoassay.

摘要

已设计出一种放射性气相色谱法来测定鱼血浆样本中的11-氧代睾酮（17β-羟基雄甾-4-烯-3,11-二酮），该方法为验证先前所述的放射免疫分析法提供了一种独立手段。通过放射性气相色谱法，利用峰高和峰重测量对雄性虹鳟鱼血浆样本中11-氧代睾酮浓度的估计分别为6.9微克/100毫升和7.4微克/100毫升，与放射免疫分析法测定的7.1微克/100毫升结果吻合良好。

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