Muronets E M
Mikrobiologiia. 1982;51(5):871-3.
A collection of stable auxotrophic mutants with a frequency of reversions to the wild type below 10(-8) was obtained from the wild strain of Rhodopseudomonas sphaeroides 2R under the action of nitrosoguanidine or nitrosomethylurea. The mutants required methionine, leucine, arginine, histidine, tryptophan and cytosine for their growth. Mutants carrying 2 or 3 auxotrophic labels in their genome were selected from the primary auxotrophs under the action of UV (lambda = 254 nm). The paper describes a semi-enriched medium in which the diameter of prototrophic colonies reaches 5 mm while that of auxotrophic colonies does not exceed 1-2 mm. The selection of microcolonies from this medium makes it possible to increase the yield of auxotrophs several times as compared with the technique of imprints.
在亚硝基胍或亚硝基甲基脲的作用下，从球形红假单胞菌2R野生菌株中获得了一组稳定的营养缺陷型突变体，其回复到野生型的频率低于10^(-8)。这些突变体生长需要甲硫氨酸、亮氨酸、精氨酸、组氨酸、色氨酸和胞嘧啶。在紫外线（λ = 254 nm）作用下，从初级营养缺陷型中筛选出基因组中带有2个或3个营养缺陷标记的突变体。本文描述了一种半富集培养基，其中原养型菌落直径可达5毫米，而营养缺陷型菌落直径不超过1 - 2毫米。与印记技术相比，从这种培养基中选择微菌落能够使营养缺陷型的产量提高数倍。
