哺乳动物微粒体对合成靛蓝中前诱变因子的代谢激活作用。

Metabolic activation of promutagenic factors in synthetic indigo by mammalian microsomes.

作者信息

Jongen W M

出版信息

Carcinogenesis. 1982;3(11):1321-3. doi: 10.1093/carcin/3.11.1321.

DOI:10.1093/carcin/3.11.1321

Abstract

The role of mammalian metabolizing enzymes in the bioactivation of promutagenic factors present in synthetic indigo has been studied in Salmonella typhimurium TA98. The activation process appears to be NADPH dependent and the enzymes involved are localized in the microsomes. Comparison of the three types of induction of rat liver homogenate showed that 3-methylcholanthrene is the most effective. Furthermore, this study revealed that 1,1'-azobis (N,N-dimethylformamide) not only oxidizes glutathione but also inhibits those microsomal enzymes that are involved in the bioactivation process.

摘要

已在鼠伤寒沙门氏菌TA98中研究了哺乳动物代谢酶在合成靛蓝中存在的前诱变因子生物活化中的作用。活化过程似乎依赖于NADPH，且所涉及的酶定位于微粒体中。对大鼠肝脏匀浆三种诱导类型的比较表明，3-甲基胆蒽最为有效。此外，该研究还揭示，1,1'-偶氮双（N,N-二甲基甲酰胺）不仅氧化谷胱甘肽，还抑制参与生物活化过程的那些微粒体酶。