一种用于测量抑制分裂抗体抑殖素的半自动微量测定方法。

A semi-automated microassay method for the measurement of ablastin, a division inhibitory antibody.

作者信息

Ferrante A

出版信息

J Immunol Methods. 1982 Nov 26;55(1):93-7. doi: 10.1016/0022-1759(82)90081-3.

Abstract

Division of Trypanosoma lewisi in the rat is terminated by ablastin, a factor(s) in the serum of rats acquired during infection which is believed to be specific antibody. In this study a semi-automated microassay technique for the measurement of ablastin is described. The system involved the use of [3H]TdR as a marker for trypanosome division and a multiple-well cell harvester to harvest trypanosomes from wells of microtitre plates. The method is versatile, quantitative and easy to perform, permitting the simultaneous measurement of numerous samples for this division-inhibitory activity.

摘要

感染期间大鼠血清中产生的一种因子（被认为是特异性抗体）抗增殖素可终止大鼠体内刘易斯锥虫的分裂。本研究描述了一种用于测定抗增殖素的半自动微量测定技术。该系统使用[3H]TdR作为锥虫分裂的标记物，并使用多孔细胞收集器从微量滴定板的孔中收集锥虫。该方法用途广泛、定量且易于操作，可同时测定大量样品的这种分裂抑制活性。