一种新的RNA聚合酶与大鼠线粒体DNA复制起点的体外转录
A new RNA polymerase and in vitro transcription of the origin of replication from rat mitochondrial DNA.
作者信息
Yaginuma K, Kobayashi M, Taira M, Koike K
出版信息
Nucleic Acids Res. 1982 Dec 11;10(23):7531-42. doi: 10.1093/nar/10.23.7531.
A new RNA polymerase was found in a rat mitochondrial extract. This enzyme showed strong template preference in vitro for the supercoiled recombinant plasmid consisting of pBR322 and the D-loop region of rat mtDNA carrying the origin of heavy-strand replication. The main products synthesized by the D-loop region were two RNAs of different sizes. Both of these products were light-strand products transcribed from the region upstream from the origin of replication. This specific transcription is discussed in relation to initiation of primer RNA synthesis for heavy-strand replication of rat mtDNA.
在大鼠线粒体提取物中发现了一种新的RNA聚合酶。这种酶在体外对由pBR322和携带重链复制起点的大鼠线粒体DNA的D环区域组成的超螺旋重组质粒表现出强烈的模板偏好。由D环区域合成的主要产物是两种不同大小的RNA。这两种产物都是从重链复制起点上游区域转录而来的轻链产物。本文讨论了这种特异性转录与大鼠线粒体DNA重链复制的引物RNA合成起始的关系。
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