大鼠视网膜中血管活性肠肽免疫反应性无长突细胞的出生后发育

Postnatal development of vasoactive intestinal polypeptide immunoreactive amacrine cells in the rat retina.

作者信息

Terubayashi H, Okamura H, Fujisawa H, Itoi M, Yanaihara N, Ibata Y

出版信息

Neurosci Lett. 1982 Dec 13;33(3):259-64. doi: 10.1016/0304-3940(82)90381-0.

Abstract

The ontogenic development of vasoactive intestinal polypeptide (VIP)-immunoreactive amacrine cells in the rat retina was studied using peroxidase-antiperoxidase (PAP) immunohistochemistry. In the rat retina, VIP immunoreactivity appeared entirely in postnatal stage. On the 12th postnatal day, VIP-immunoreactive amacrine cells could be first detected. However, VIP immunoreactivity was very weak and VIP-immunoreactive amacrine cell processes could not be observed at this stage. On the 21st postnatal day, VIP-immunoreactive amacrine cell bodies became more mature and their processes were distinctly observed, resembling their appearance in adult rat retinas. VIP immunoreactivity could be detected in both stratified and diffuse amacrine cells at this stage. The relationship between the first appearance of VIP-immunoreactive amacrine cells and the synaptic formation in the inner plexiform layer is briefly discussed.

摘要

采用过氧化物酶抗过氧化物酶（PAP）免疫组织化学方法，研究了大鼠视网膜中血管活性肠多肽（VIP）免疫反应性无长突细胞的个体发生发育。在大鼠视网膜中，VIP免疫反应性完全出现在出生后阶段。在出生后第12天，首次检测到VIP免疫反应性无长突细胞。然而，此时VIP免疫反应性非常弱，且在此阶段未观察到VIP免疫反应性无长突细胞的突起。在出生后第21天，VIP免疫反应性无长突细胞体变得更加成熟，其突起清晰可见，类似于成年大鼠视网膜中的外观。在此阶段，在分层和弥散性无长突细胞中均能检测到VIP免疫反应性。简要讨论了VIP免疫反应性无长突细胞的首次出现与内网状层突触形成之间的关系。