Munker R, Stünkel K, Thierfelder S
Biomed Pharmacother. 1982;36(4):210-2.
The phenomenon of autorosette formation was investigated by incubating the peripheral blood mononuclear cells of 5 normal persons with a series of monoclonal antibodies and an FITC-labelled second layer before allowing them to bind to autologous red blood cells. The antibodies used were OKT3, OKT4, OKT5, OKM1 and OKIa1, defining mature T cells, the inducer and suppressor subsets of T cells, monocytes and Ia antigen bearing cells, respectively. By this procedure the percentage of autorosette-forming cells was only slightly decreased. With this double marker analysis, the percentage of fluorescent cells among cells forming autologous rosettes was approximately the same as in the total mononuclear cell population before rosetting. In conclusion we could demonstrate that autologous rosettes in man may be formed by T and probably B lymphocytes as well as by monocytes and that there is no preference for the inducer and suppressor subsets of T lymphocytes.
通过将5名正常人的外周血单个核细胞与一系列单克隆抗体及异硫氰酸荧光素标记的第二层抗体一起孵育，然后使其与自体红细胞结合，对自身玫瑰花结形成现象进行了研究。所使用的抗体为OKT3、OKT4、OKT5、OKM1和OKIa1，它们分别界定成熟T细胞、T细胞的诱导亚群和抑制亚群、单核细胞以及表达Ia抗原的细胞。通过该程序，自身玫瑰花结形成细胞的百分比仅略有下降。通过这种双重标记分析，在形成自体玫瑰花结的细胞中荧光细胞的百分比与玫瑰花结形成前总单核细胞群体中的大致相同。总之，我们可以证明，人类的自体玫瑰花结可能由T淋巴细胞、可能还有B淋巴细胞以及单核细胞形成，并且对T淋巴细胞的诱导亚群和抑制亚群没有偏好。
