Pederson R A, Buchan A M, Zahedi-Asl S, Chan C B, Brown J C
Regul Pept. 1982 Dec;5(1):53-63. doi: 10.1016/0167-0115(82)90075-1.
The effect of jejunoileal bypass (JIB) on the enteroinsular axis was studied in vivo and in vitro in the rat. Glucose, insulin and GIP responses to oral glucose were compared in JIB and control rats. The effect of glucose and GIP on insulin release from the isolated perfused pancreas of the same animals was investigated to determine if JIB altered the sensitivity of the beta cell. Immunocytochemical studies of gut and pancreas were also carried out. Glucose, insulin and GIP responses to a glucose load were blunted after JIB, although basal GIP levels were elevated in these animals. The insulin response of the perfused JIB pancreas to GIP was 70% reduced from controls although the insulin response to glucose appeared normal. The size and area of JIB islets were unchanged from controls as was the distribution of insulin, glucagon, somatostatin and pancreatic polypeptide. GIP immunoreactive cells were present in all regions of the intestine including the JIB blind loop. This study confirms the findings of others that a relationship exists between reduced GIP and insulin response to oral glucose after JIB, and indicates that a decrease in sensitivity of the beta cell to GIP occurs following JIB that is not rapidly reversible. GIP secreted from blind loop mucosa may contribute to the high basal GIP found in JIB rats and may be causally connected to the fall in beta cell sensitivity.
在大鼠体内和体外研究了空肠回肠旁路术（JIB）对肠胰岛轴的影响。比较了JIB大鼠和对照大鼠对口服葡萄糖的葡萄糖、胰岛素和胃抑肽（GIP）反应。研究了葡萄糖和GIP对同一动物离体灌注胰腺胰岛素释放的影响，以确定JIB是否改变了β细胞的敏感性。还对肠道和胰腺进行了免疫细胞化学研究。JIB后，对葡萄糖负荷的葡萄糖、胰岛素和GIP反应减弱，尽管这些动物的基础GIP水平升高。灌注的JIB胰腺对GIP的胰岛素反应比对照降低了70%，尽管对葡萄糖的胰岛素反应似乎正常。JIB胰岛的大小和面积与对照相比没有变化，胰岛素、胰高血糖素、生长抑素和胰多肽的分布也是如此。GIP免疫反应性细胞存在于包括JIB盲袢在内的肠道所有区域。这项研究证实了其他人的发现，即JIB后GIP降低与口服葡萄糖的胰岛素反应之间存在关系，并表明JIB后β细胞对GIP的敏感性降低，且这种降低不是快速可逆的。盲袢黏膜分泌的GIP可能导致JIB大鼠基础GIP升高，并且可能与β细胞敏感性下降存在因果关系。
