Ilinov P, Nedelcheva I, Goranov I
Vutr Boles. 1978;17(3):87-95.
A method is presented for the determination of serum lipid groups changes in conditions of venous fatty tolerance test as well as for time defining in reaching the initial levels of serum triglycerides. A variant of the quantitative thin-layer chromatography is applied, combined with carbonization and densitometry for lipid groups determination in the serum of 40 patients, subdivided into four groups: with chronic ischemic heart disease, with arterial hypertension, with obesity and a control ggroup. Lipid analysis was carried out by the 5th, 20th and 40th minute after the beginning of the test. After loading with Intralipid, it was established to develop quantitative changes not only in triglycerides but in the rest of the lipid groups as well. At the same time, the time for reaching the initial values of triglycerides, calculated by extrapolation, shows significant differences between the control group and those with ischemic heart disease and arterial hypertension. The investigation method adopted guarantees more thorough information about lipid changes after Intralipid infection, than the method with the determination of plasma opasity in corresponding time intervals. The criterion "time for reaching the initial values" characterized more fully the dynamic changes in triglycerides and could be used in the detection of latent anomalies in lipid metabolism.
本文介绍了一种用于测定静脉脂肪耐量试验条件下血清脂质组变化以及确定血清甘油三酯达到初始水平所需时间的方法。应用了定量薄层色谱的一种变体，结合碳化和密度测定法，对40例患者的血清脂质组进行测定，这些患者分为四组：患有慢性缺血性心脏病、动脉高血压、肥胖症以及一个对照组。在试验开始后的第5分钟、第20分钟和第40分钟进行脂质分析。在输注英脱利匹特后发现，不仅甘油三酯，其他脂质组也发生了定量变化。同时，通过外推法计算得出的甘油三酯达到初始值的时间显示，对照组与患有缺血性心脏病和动脉高血压的组之间存在显著差异。所采用的研究方法比在相应时间间隔内测定血浆浊度的方法能保证更全面地了解英脱利匹特输注后脂质的变化情况。“达到初始值的时间”这一标准更充分地体现了甘油三酯的动态变化，可用于检测脂质代谢中的潜在异常。
