肾素在小鼠脑内的免疫组织化学定位

Immunohistochemical localization of renin in mouse brain.

作者信息

Inagami T, Clemens D L, Celio M R, Brown A, Sandru L, Herschkowitz N, Hoffman L H, Kasselberg A G

出版信息

Neurosci Lett. 1980 May 15;18(1):91-8. doi: 10.1016/0304-3940(80)90218-9.

Abstract

Immunohistochemical studies of mouse brain by the peroxidase-antiperoxidase method using monospecific antimouse renin antibodies has revealed the intracellular localization of renin in stellate and small ovoid cells. Renin-containing ovoid cells were observed in the spinal cord, medulla oblongata, pons, granular layers of the cerebellum, deep cerebellar region, and the lamina terminalis; whereas immunostainable stellate cells were found in the cerebral cortex, hippocampus, dentate gyrus and septum. Intracellular localization of renin rather than intravascular localization supports an endogenous origin of this enzyme in the brain. Wide distribution in different types of cells suggests different types of regulatory mechanisms. Double immunostaining with antigalactocerebroside and antirenin antibodies indicates the presence of renin in oligodendrocytes.

摘要

使用单特异性抗小鼠肾素抗体，通过过氧化物酶-抗过氧化物酶法对小鼠脑进行免疫组织化学研究，揭示了肾素在星状细胞和小卵圆形细胞中的细胞内定位。在脊髓、延髓、脑桥、小脑颗粒层、小脑深部区域和终板中观察到含肾素的卵圆形细胞；而在大脑皮层、海马体、齿状回和隔膜中发现了可免疫染色的星状细胞。肾素的细胞内定位而非血管内定位支持了该酶在脑中的内源性起源。在不同类型细胞中的广泛分布表明存在不同类型的调节机制。用抗半乳糖脑苷脂和抗肾素抗体进行双重免疫染色表明少突胶质细胞中存在肾素。