Yoshikai Y, Miake S, Mituyama M, Nomoto K
J Gen Microbiol. 1982 Dec;128(12):2857-63. doi: 10.1099/00221287-128-12-2857.
The contribution of activated macrophages to protection against Escherichia coli was studied in mice. Mice treated intraperitoneally with killed Corynebacterium parvum organisms 1 d prior to challenge showed an increased resistance to intraperitoneal infection with E. coli; the predominant leucocytes in the peritoneal cavity of these animals were polymorphonuclear cells. However, treatment with C. parvum 4 d prior to challenge induced mainly activated macrophages in the peritoneal cavity and host resistance to the infection was not increased. Activated macrophages from such mice showed both enhanced phagocytic activity in vivo and a high degree of intracellular killing of E. coli in vitro. At the same time these cells became more susceptible to the cytotoxic effect of endotoxin. After challenge with E. coli there was a marked decrease in the number of peritoneal macrophages in mice that were treated with C. parvum 4 d prior to challenge. Increased susceptibility of activated macrophages to the cytotoxic effect of endotoxin could explain the absence of enhanced resistance to E. coli infection in such animals.
在小鼠中研究了活化巨噬细胞对抵抗大肠杆菌的作用。在受到攻击前1天经腹腔注射灭活短小棒状杆菌的小鼠，对腹腔感染大肠杆菌表现出增强的抵抗力；这些动物腹腔中的主要白细胞是多形核细胞。然而，在受到攻击前4天用短小棒状杆菌处理，主要诱导腹腔中的活化巨噬细胞，且宿主对感染的抵抗力并未增强。来自此类小鼠的活化巨噬细胞在体内表现出增强的吞噬活性，在体外对大肠杆菌具有高度的细胞内杀伤作用。同时，这些细胞对内毒素的细胞毒性作用变得更敏感。在用短小棒状杆菌在受到攻击前4天处理的小鼠中，受到大肠杆菌攻击后腹腔巨噬细胞数量明显减少。活化巨噬细胞对内毒素细胞毒性作用敏感性增加，这可以解释此类动物对大肠杆菌感染抵抗力未增强的原因。
