Takeda Y, Yoshikai Y, Ohga S, Nomoto K
Department of Immunology, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan.
Immunopharmacol Immunotoxicol. 1990;12(3):457-77. doi: 10.3109/08923979009006473.
Protection against Listeria monocytogenes and Escherichia coli in mice was enhanced by an intraperitoneal (i.p.) administration of polysaccharide "RBS". Peritoneal macrophages from mice administered i.p. with 30 mg/kg doses of RBS 4 days earlier exhibited increased scavenger functions as assessed by in vivo phagocytosis, in vitro intracellular killing and generation of superoxide anion. When cytokine production of the macrophages was assessed by biological assay and Northern blotting analysis, interleukin (IL)-1 production and IL-1 alpha gene expression were significantly increased in macrophages from RBS-treated mice. On the other hand, tumor necrosis factor (TNF) alpha gene was expressed in macrophages from RBS-treated mice at a much reduced level as compared with those in mice treated i.p. with Corynebacterium parvum on 4 days earlier. In correlation with expression of TNF gene in the macrophages, RBS-treated mice were less susceptible to the lethal toxicity of LPS than C.parvum-treated mice. In RBS-treated mice, in vivo elimination of bacteria was enhanced at the early phase of infection with L.monocytogenes or E.coli, resulting in augmentation of host defense against these bacterial infection. These results suggest that adequately enhanced activities of macrophages acting as scavenger phagocytes play important roles in the enhanced resistance against bacteria in mice treated i.p. with RBS.
腹腔注射多糖“RBS”可增强小鼠对单核细胞增生李斯特菌和大肠杆菌的抵抗力。4天前腹腔注射30mg/kg剂量RBS的小鼠腹腔巨噬细胞，通过体内吞噬作用、体外细胞内杀伤和超氧阴离子生成评估，其清除功能增强。当通过生物学检测和Northern印迹分析评估巨噬细胞的细胞因子产生时，RBS处理小鼠的巨噬细胞中白细胞介素（IL）-1的产生和IL-1α基因表达显著增加。另一方面，与4天前腹腔注射微小棒状杆菌的小鼠相比，RBS处理小鼠的巨噬细胞中肿瘤坏死因子（TNF）α基因的表达水平大大降低。与巨噬细胞中TNF基因的表达相关，RBS处理的小鼠比微小棒状杆菌处理的小鼠对LPS的致死毒性更不敏感。在RBS处理的小鼠中，单核细胞增生李斯特菌或大肠杆菌感染早期细菌的体内清除增强，从而增强了宿主对这些细菌感染的防御能力。这些结果表明，作为清除吞噬细胞的巨噬细胞活性的充分增强，在腹腔注射RBS处理的小鼠对细菌抵抗力增强中起重要作用。
