Gilbert P, Kettenmann H, Orkand R K, Schachner M
Neurosci Lett. 1982 Dec 30;34(2):123-8. doi: 10.1016/0304-3940(82)90163-x.
SITS, a stilbene isothiocyanate derivative, was injected by iontophoresis into neurons and oligodendrocytes in cultures of mouse spinal cord. The dye readily stains the entire cell. When excited with ultraviolet light it emits a blue fluorescence. Thus, SITS-injected cells may be distinguished from those injected with Lucifer yellow CH. Furthermore, the blue cells can be identified in culture after the cells have been labeled with two different antibodies using rhodamine (TRITC) or fluorescein (FITC) as fluorochromes by the use of appropriate filter combinations.
SITS是一种芪异硫氰酸酯衍生物，通过离子电渗法注入小鼠脊髓培养物中的神经元和少突胶质细胞。该染料很容易将整个细胞染色。用紫外光激发时，它会发出蓝色荧光。因此，注射了SITS的细胞可以与注射了路西法黄CH的细胞区分开来。此外，在细胞用罗丹明（TRITC）或荧光素（FITC）作为荧光染料标记了两种不同抗体后，可以通过使用合适的滤光片组合在培养物中识别出蓝色细胞。
