Boyd A, Mandel G, Simon M I
Symp Soc Exp Biol. 1982;35:123-37.
Several of the gene products required for bacterial motility and chemotaxis are integral components of the cytoplasmic membrane. Amplification of the expression of one of these genes, motB, using in vitro recombinant DNA techniques, has allowed us to initiate a study of the assembly of this protein into the membrane. The identical sizes of the motB gene products synthesised in vivo and in vitro strongly suggest that assembly is achieved without the involvement of a proteolytically processed leader peptide. Further analysis of this system should yield valuable insights into the specific determinants of the cellular localisation of the motB gene product. Two other membrane proteins, the tsr and tar products have been studied in relation to their role as integrating channels for the transfer of sensory information into the bacterial cell. A model of the functional properties of these proteins is presented and discussed in terms of recent findings that they are subject to multiple covalent modifications during sensory adaptation.
细菌运动性和趋化性所需的几种基因产物是细胞质膜的组成成分。利用体外重组DNA技术扩增其中一个基因motB的表达，使我们能够启动对该蛋白组装到膜中的研究。在体内和体外合成的motB基因产物大小相同，这强烈表明组装过程无需经过蛋白水解加工的前导肽参与。对该系统的进一步分析应能为motB基因产物细胞定位的特定决定因素提供有价值的见解。另外两种膜蛋白，即tsr和tar产物，已就其作为将感官信息传递到细菌细胞中的整合通道的作用进行了研究。根据最近发现它们在感官适应过程中会发生多种共价修饰这一结果，提出并讨论了这些蛋白功能特性的模型。
