Lewin R A, Lee T H, Fang L S
Symp Soc Exp Biol. 1982;35:421-37.
Over 200 strains of green algal flagellates, representing about 100 species, were examined for their suitability as experimental organisms for studies of flagellar activity. The cells of all species shed their flagella under unfavourable conditions of temperature or pH, or in the presence of alcohols, detergents or toxic agents of various kinds. For further studies of flagellar activity, motility and autotomy (biologically induced shedding) in particular, we selected four species of Chlamydomonas: C. dysosmos Moewus, C. moewusii Gerloff, C. monoica Strehlow and C. reinhardtii Dangeard. Agents found to inhibit motility without inducing death or flagellar autotomy included azide, arsenite, thiosulphate, cyanide, ferricyanide, hydroxylamine, chloral hydrate, malonate, p-chloro-mercury benzoate and cytochalasin-B, each in a limited range of concentrations which differed according to species and strain. Higher concentrations of these agents caused the flagella to be shed. Since flagellar autotomy is a means by which a cell can quickly reduce the area of its permeable surface, it may have a positive survival value for species liable to be subjected to unfavourable physicochemical conditions.
为研究鞭毛活性，我们检测了200多种绿藻鞭毛虫（约100个物种）作为实验生物的适用性。在温度或pH值不利的条件下，或存在酒精、洗涤剂或各种有毒物质时，所有物种的细胞都会脱落鞭毛。为进一步研究鞭毛活性，特别是运动性和自切（生物诱导脱落），我们选择了四种衣藻：恶臭衣藻（C. dysosmos Moewus）、莫氏衣藻（C. moewusii Gerloff）、单生衣藻（C. monoica Strehlow）和莱茵衣藻（C. reinhardtii Dangeard）。发现能抑制运动但不导致死亡或鞭毛自切的物质包括叠氮化物、亚砷酸盐、硫代硫酸盐、氰化物、铁氰化物、羟胺、水合氯醛、丙二酸盐、对氯汞苯甲酸和细胞松弛素B，每种物质在有限的浓度范围内起作用，浓度范围因物种和菌株而异。这些物质浓度较高时会导致鞭毛脱落。由于鞭毛自切是细胞快速减少其可渗透表面积的一种方式，对于易受不利物理化学条件影响的物种来说，它可能具有积极的生存价值。
