[体外集落测定（人肿瘤干细胞测定）中实体瘤的集落形成]

[Colony formation of solid tumors in in vitro colony assay (human tumor stem cell assay)].

作者信息

Inoue K, Arakawa M, Ogawa M, Inagaki J, Horikoshi N, Ezaki K, Aiba K, Domyo M, Miyamoto H, Ikeda K

出版信息

Gan To Kagaku Ryoho. 1982 Dec;9(12):2128-32.

Abstract

Colony forming ability of solid tumor cells was studied in a tumor colony assay (human tumor stem cell assay). In 50 cases of solid tumors, cloning efficiencies of 5 X 10(5) cells plated were as follows: breast cancer 12/12 (100%), colon cancer 10/11 (91%), ovarian cancer 9/9 (100%), sarcomas 7/9 (78%), gastric cancer 3/6 (50%), endometrial cancer 2/2 and pancreatic cancer 1/1. An overall cloning efficiency was 88% (44/50) and this rate is higher than those reported in literatures. Ovarian cancer showed the highest plating efficiency of 0.07% (number of colonies/number of cells plated X 100%) in various solid tumors tested. Subsequently, plating efficiencies of colon and breast cancer were 0.03 and 0.01%, respectively. In the cases of sarcomas and gastric cancer, low plating efficiencies were seen (0.008%, 0.003%). The overall rate succeeded colony growth of solid tumors was somewhat higher in enzymatically treated tumor cells, that is, cloning efficiencies in mechanical and enzymatic methods were 85 and 90%, respectively. The enzymatic disaggregation is an advantageous method in gastric cancer and sarcomas. Various solid tumors can be formed colonies in soft agar and chemosensitivity test using in vitro colony assay is expected in solid tumors.

摘要

在肿瘤集落测定法（人肿瘤干细胞测定法）中研究了实体瘤细胞的集落形成能力。在50例实体瘤中，接种5×10⁵个细胞的克隆效率如下：乳腺癌12/12（100%），结肠癌10/11（91%），卵巢癌9/9（100%），肉瘤7/9（78%），胃癌3/6（50%），子宫内膜癌2/2，胰腺癌1/1。总体克隆效率为88%（44/50），该比率高于文献报道的比率。在测试的各种实体瘤中，卵巢癌的接种效率最高，为0.07%（集落数/接种细胞数×100%）。随后，结肠癌和乳腺癌的接种效率分别为0.03%和0.01%。在肉瘤和胃癌病例中，接种效率较低（0.008%，0.003%）。酶处理的肿瘤细胞中实体瘤成功集落生长的总体比率略高，即机械法和酶法的克隆效率分别为85%和90%。酶解在胃癌和肉瘤中是一种有利的方法。各种实体瘤可在软琼脂中形成集落，预计可利用体外集落测定法进行实体瘤的化学敏感性试验。