Blake D A, DiBlasi M C, Gordon G B
Fundam Appl Toxicol. 1981 Nov-Dec;1(6):415-8. doi: 10.1016/s0272-0590(81)80019-x.
Trifluoroethanol, trifluoroacetaldehyde and trifluoroacetate were found to have no mutagenic activity in the standard Salmonella typhimurium reverse mutation assay (Ames test) using a closed incubation system. Negative results were also obtained when incubation mixtures included 9000 x g supernatant fractions of rat liver or testes homogenates along with an NADPH generating system. Rats were pretreated with a polychlorinated biphenyl mixture to induce biotransforming enzyme activity. These results suggest that the previously reported mutagenic activity of fluroxene is not due to metabolites arising from the trifluoroethyl side of the molecule and that inhibition of spermatogenesis in rats by trifluoroethanol is not mediated through a mutagenic mechanism.
在使用封闭培养系统的标准鼠伤寒沙门氏菌回复突变试验（艾姆斯试验）中，发现三氟乙醇、三氟乙醛和三氟乙酸盐无致突变活性。当培养混合物中包含大鼠肝脏或睾丸匀浆的9000×g上清液组分以及NADPH生成系统时，也得到了阴性结果。用多氯联苯混合物对大鼠进行预处理以诱导生物转化酶活性。这些结果表明，先前报道的氟烯的致突变活性并非源于分子三氟乙基侧产生的代谢物，并且三氟乙醇对大鼠精子发生的抑制作用不是通过诱变机制介导的。
