Joseph S W, Eggena D W, Danusantoso H, Tanzil H O, Chatim A
Southeast Asian J Trop Med Public Health. 1976 Dec;7(4):513-7.
Sputum specimens from more than 1000 Indonesian tuberculosis suspects were examined by bacteriologic culture, and by bright field and fluorescence microscopy. Two hundred twenty had positive Mycobacterium tuberculosis cultures and of these 68% were positive by fluorescence microscopy. Agreement between culture, both negative and positive, and fluorescence microscopy was 87%. Sensitivity to antituberculous drugs was performed in 209 isolates. Significant resistance to isoniazid, para-amino salicylic acid, and streptomycin was found i.e. 65 (31%), 19 (19%), 54 (26%), respectively. Fluorescence microscopy was a useful method for rapid microscopic confirmation of tuberculosis and was especially valuable in detecting difficult-to-culture organisms.
对1000多名印度尼西亚结核病疑似患者的痰液标本进行了细菌学培养、明视野显微镜检查和荧光显微镜检查。220例结核分枝杆菌培养呈阳性，其中68%的病例荧光显微镜检查呈阳性。培养结果（包括阴性和阳性）与荧光显微镜检查的一致性为87%。对209株分离菌进行了抗结核药物敏感性检测。发现对异烟肼、对氨基水杨酸和链霉素有显著耐药性，分别为65株（31%）、19株（19%）、54株（26%）。荧光显微镜检查是快速显微镜确诊结核病的一种有用方法，在检测难以培养的微生物方面尤其有价值。
