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[使用单一试剂比色法测定与去铁胺B螯合的尿铁]

[Colorimetric determination of urinary iron, chelated with deferoxamine B, using a single reagent].

作者信息

Grisler R, Ferrari M, Cantù Rajnoldi A, Ravazzani V

出版信息

Quad Sclavo Diagn. 1982 Dec;18(4):383-91.

PMID:6764669
Abstract

A new colorimetric method for the determination of urinary iron chelated with desferrioxamine B is proposed. A single reagent (ferrozine plus thioglycolic acid 1%) is utilized. Analytical range reached about 25 mg of iron/l, while the detection limit was 0.2 p.p.m. The evaluation of imprecision and inaccuracy were within acceptable limits. Moreover, there is good correlation with the atomic absorption spectrophotometric technique. This test shows good reliability.

摘要

提出了一种测定与去铁胺B螯合的尿铁的新比色法。使用单一试剂(亚铁嗪加1%巯基乙酸)。分析范围达到约25毫克铁/升,而检测限为0.2 ppm。精密度和准确度评估在可接受范围内。此外,与原子吸收分光光度法有良好的相关性。该试验显示出良好的可靠性。

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