[Comparative studies in vitro of the toxic effect of water extracts from synthetic materials in biological tests on living cells].

Synthetic materials for medicine and pharmacy described as medical grade materials are constantly improved and new materials appear every year. There are various kinds of synthetic materials within the same group and they differ in qualitative and quantitative choice of chemical compounds used for their synthesis, and in technology of their production. These factors influence physico-chemical and biological properties of the materials. There is also a possibility of qualitative changes in synthetic products caused by depolimerization and migration of chemical compounds which depends on the conditions and time of their storage, and on the method of sterilization. The production of a new synthetic item is connected with the necessity of selective choice of the proper material. The choice is made on the basis of complex physico-chemical and biological investigations. Systematic controls of multimolecular materials after polimerizations and controls of ready products should be made in the course of series production. Controls of toxicity are usually made according to chosen one or few biological testing methods in vitro. The results of parallel investigations performed according to different methods are not sufficient for univocal evaluation of the material since they can be either divergent or contradictory. Therefore new tests and expanding methods of biological investigations are constantly looked for. A new technique of synthetic materials toxicity evaluation with the fresh diluted bulls semen has been developed after for several years, of research. The results indicated that the bulls semen was useful in toxicity evaluation. This present work constitutes a continuation and recapitulation of the investigations which had the following goals to be attained. 1. Improvement of investigations technique, development of the criteria of results evaluation and presentation of the basic principles of our method of toxicity investigation of synthetic materials water extracts on the bull semen. 2. Evaluation of the toxicity of various synthetic products used in animals insemination and biomedical products on bull semen. 3. The comparison of the results of toxicity investigation of synthetic materials water extracts on bulls semen with the results of investigations performed on cell cultures HF, mouse cells L929, chicken embryo cells CEC and the results of hemolytic test. 4. The complex evaluation of the own method of toxicity investigation of synthetic materials water extracts with the application of the fresh bulls semen. 55 various materials and products used in medicine, in animals insemination and in the food industry were chosen for the investigation.(ABSTRACT TRUNCATED AT 400 WORDS)