兔前脑胆碱乙酰转移酶的免疫组织化学定位

Immunohistochemical localization of choline acetyltransferase in rabbit forebrain.

作者信息

Chao L P, Kan K S, Hung F M

出版信息

Brain Res. 1982 Mar 4;235(1):65-82. doi: 10.1016/0006-8993(82)90196-2.

Abstract

uinea pig antiserum specific to the purified bovine choline acetyltransferase was used to demonstrate the localization of this enzyme in rabbit forebrain by the peroxidase-antiperoxidase immunohistochemical method. Choline acetyltransferase was localized in olfactory bulb, olfactory tract, olfactory tubercle, piriform cortex, septum, diagonal band, basal ganglia, thalamus, hypothalamus, subthalamus, habenula, cerebral cortex, hippocampal region, corpus callosum, internal capsule, fornix, longitudinal striae and other areas. The findings reflect the distribution of cholinergic axons and, possibly, their terminals. These observations correlate well with biochemical determinations of choline acetyltransferase and with previously proposed cholinergic pathways.

摘要

用针对纯化牛胆碱乙酰转移酶的豚鼠抗血清，通过过氧化物酶-抗过氧化物酶免疫组织化学方法来证明该酶在兔前脑的定位。胆碱乙酰转移酶定位于嗅球、嗅束、嗅结节、梨状皮质、隔区、斜角带、基底神经节、丘脑、下丘脑、下丘脑底部、缰核、大脑皮质、海马区、胼胝体、内囊、穹窿、纵纹及其他区域。这些发现反映了胆碱能轴突及其可能的终末的分布。这些观察结果与胆碱乙酰转移酶的生化测定以及先前提出的胆碱能通路密切相关。