核黄素对培养的神经母细胞瘤细胞中单胺氧化酶活性的影响。
Effect of riboflavin on monoamine oxidase activity in cultured neuroblastoma cells.
作者信息
Bonnefil V, Castiglione C M, Cawthon R M, Breakefield X O
机构信息
Department of Human Genetics, Yale University School of Medicine, New Haven, Connecticut 06510.
出版信息
Cell Mol Neurobiol. 1981 Dec;1(4):351-9. doi: 10.1007/BF00716270.
Abstract
Monoamine oxidase (MAO) depends on a covalently attached FAD cofactor for activity. Activity is depressed in mouse neuroblastoma cells (N1E-115) grown in synthetic N2 medium lacking riboflavin. MAO activity in depleted cells is stimulated by added riboflavin, and this recovery is blocked by inhibitors of RNA and protein synthesis, and not by an inhibitor of protein glycosylation Recovery from riboflavin depletion appears to depend upon new RNA and protein synthesis, and not on the addition of FAD cofactor to an inactive MAO precursor.
摘要
单胺氧化酶（MAO）的活性依赖于共价连接的黄素腺嘌呤二核苷酸（FAD）辅因子。在缺乏核黄素的合成N2培养基中培养的小鼠神经母细胞瘤细胞（N1E-115）中，MAO的活性受到抑制。添加核黄素可刺激耗尽细胞中的MAO活性，并且这种恢复被RNA和蛋白质合成抑制剂所阻断，而不是被蛋白质糖基化抑制剂所阻断。从核黄素耗竭中的恢复似乎取决于新的RNA和蛋白质合成，而不是向无活性的MAO前体添加FAD辅因子。
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