核黄素对培养的神经母细胞瘤细胞中单胺氧化酶活性的影响。
Effect of riboflavin on monoamine oxidase activity in cultured neuroblastoma cells.
作者信息
Bonnefil V, Castiglione C M, Cawthon R M, Breakefield X O
机构信息
Department of Human Genetics, Yale University School of Medicine, New Haven, Connecticut 06510.
出版信息
Cell Mol Neurobiol. 1981 Dec;1(4):351-9. doi: 10.1007/BF00716270.
Abstract
Monoamine oxidase (MAO) depends on a covalently attached FAD cofactor for activity. Activity is depressed in mouse neuroblastoma cells (N1E-115) grown in synthetic N2 medium lacking riboflavin. MAO activity in depleted cells is stimulated by added riboflavin, and this recovery is blocked by inhibitors of RNA and protein synthesis, and not by an inhibitor of protein glycosylation Recovery from riboflavin depletion appears to depend upon new RNA and protein synthesis, and not on the addition of FAD cofactor to an inactive MAO precursor.
摘要
单胺氧化酶（MAO）的活性依赖于共价连接的黄素腺嘌呤二核苷酸（FAD）辅因子。在缺乏核黄素的合成N2培养基中培养的小鼠神经母细胞瘤细胞（N1E-115）中，MAO的活性受到抑制。添加核黄素可刺激耗尽细胞中的MAO活性，并且这种恢复被RNA和蛋白质合成抑制剂所阻断，而不是被蛋白质糖基化抑制剂所阻断。从核黄素耗竭中的恢复似乎取决于新的RNA和蛋白质合成，而不是向无活性的MAO前体添加FAD辅因子。
相似文献
1
Effect of riboflavin on monoamine oxidase activity in cultured neuroblastoma cells.核黄素对培养的神经母细胞瘤细胞中单胺氧化酶活性的影响。
Cell Mol Neurobiol. 1981 Dec;1(4):351-9. doi: 10.1007/BF00716270.
2
The FAD binding sites of human liver monoamine oxidases A and B: investigation of the role of flavin ribityl side chain hydroxyl groups in the covalent flavinylation reaction and catalytic activities.人肝脏单胺氧化酶A和B的黄素腺嘌呤二核苷酸（FAD）结合位点：黄素核醇侧链羟基在共价黄素化反应和催化活性中作用的研究
Biochim Biophys Acta. 2000 Jan 3;1476(1):27-32. doi: 10.1016/s0167-4838(99)00222-8.
3
Influence of FAD structure on its binding and activity with the C406A mutant of recombinant human liver monoamine oxidase A.黄素腺嘌呤二核苷酸（FAD）结构对其与重组人肝脏单胺氧化酶A的C406A突变体结合及活性的影响
J Biol Chem. 2000 Jul 7;275(27):20527-32. doi: 10.1074/jbc.M002132200.
4
Flavinylation of monoamine oxidase B.单胺氧化酶B的黄素化
J Biol Chem. 1995 Oct 6;270(40):23653-60. doi: 10.1074/jbc.270.40.23653.
5
Brain monoamine oxidase and replacement of its coenzyme flavin in rats.
J Nutr. 1981 Aug;111(8):1397-402. doi: 10.1093/jn/111.8.1397.
6
Expression of A and B types of monoamine oxidase in differentiated neuroblastoma hybrid cells.
J Neurochem. 1985 Mar;44(3):755-8. doi: 10.1111/j.1471-4159.1985.tb12879.x.
7
Influence of flavin analogue structure on the catalytic activities and flavinylation reactions of recombinant human liver monoamine oxidases A and B.黄素类似物结构对重组人肝脏单胺氧化酶A和B的催化活性及黄素化反应的影响
J Biol Chem. 1999 Aug 13;274(33):23515-25. doi: 10.1074/jbc.274.33.23515.
8
Monoamine oxidase: synthesis, metabolism and function.
Mod Probl Pharmacopsychiatry. 1983;19:1-14. doi: 10.1159/000407499.
9
R-(-)-Deprenyl inhibits monocytic THP-1 cell neurotoxicity independently of monoamine oxidase inhibition.R-(-)-司来吉兰可独立于单胺氧化酶抑制作用之外，抑制单核细胞THP-1细胞的神经毒性。
Exp Neurol. 2000 Dec;166(2):458-64. doi: 10.1006/exnr.2000.7517.
10
Arginine-42 and threonine-45 are required for FAD incorporation and catalytic activity in human monoamine oxidase B.精氨酸-42和苏氨酸-45是人类单胺氧化酶B中黄素腺嘌呤二核苷酸（FAD）掺入和催化活性所必需的。
Biochemistry. 1998 Sep 1;37(35):12360-6. doi: 10.1021/bi9806910.
引用本文的文献
1
Alterations in the tyrosine and phenylalanine pathways revealed by biochemical profiling in cerebrospinal fluid of Huntington's disease subjects.通过对亨廷顿病患者脑脊液的生化特征分析揭示酪氨酸和苯丙氨酸代谢途径的改变。
Sci Rep. 2019 Mar 11;9(1):4129. doi: 10.1038/s41598-019-40186-5.
2
Monoamine oxidases A and B are differentially regulated by glucocorticoids and "aging" in human skin fibroblasts.单胺氧化酶A和B在人皮肤成纤维细胞中受到糖皮质激素和“衰老”的差异调节。
Cell Mol Neurobiol. 1986 Jun;6(2):121-50. doi: 10.1007/BF00711066.
本文引用的文献
1
A SENSITIVE AND SPECIFIC ASSAY FOR THE ESTIMATION OF MONOAMINE OXIDASE.一种用于估算单胺氧化酶的灵敏且特异的检测方法。
Biochem Pharmacol. 1963 Dec;12:1439-41. doi: 10.1016/0006-2952(63)90215-6.
2
An electron-transport system associated with the outer membrane of liver mitochondria. A biochemical and morphological study.与肝线粒体外膜相关的电子传递系统。一项生化与形态学研究。
J Cell Biol. 1967 Feb;32(2):415-38. doi: 10.1083/jcb.32.2.415.
3
Properties of monoamine oxidase in control and Lesch-Nyhan fibroblasts.对照和莱施-奈恩成纤维细胞中单胺氧化酶的特性
Biochem Genet. 1980 Jun;18(5-6):577-90. doi: 10.1007/BF00484403.
4
Monoamine oxidase A and B activities in liver of riboflavin-deficient rats.
Biochem Pharmacol. 1980 Oct 1;29(19):2693-4. doi: 10.1016/0006-2952(80)90090-8.
5
Iron content and spectral properties of highly purified bovine liver monoamine oxidase.高纯度牛肝单胺氧化酶的铁含量及光谱特性
Arch Biochem Biophys. 1981 Nov;212(1):147-53. doi: 10.1016/0003-9861(81)90353-2.
6
Differences in the structure of A and B forms of human monoamine oxidase.人类单胺氧化酶A和B形式的结构差异。
J Neurochem. 1981 Aug;37(2):363-72. doi: 10.1111/j.1471-4159.1981.tb00464.x.
7
Dexamethasone selectively increases monoamine oxidase type A in human skin fibroblasts.
Biochem Biophys Res Commun. 1981 Feb 12;98(3):836-43. doi: 10.1016/0006-291x(81)91187-6.
8
Exposure of mitochondrial outer membranes to neuraminidase selectively destroys monoamine oxidase A activity.
J Pharm Pharmacol. 1980 Jan;32(1):65-6. doi: 10.1111/j.2042-7158.1980.tb12850.x.
9
A new method for serum iron and total iron-binding capacity by atomic absorption spectrophotometry.一种用原子吸收分光光度法测定血清铁和总铁结合力的新方法。
Clin Chem. 1969 Jun;15(6):438-44.
10
The labeling in vivo of monoamine oxidase by 14 C-pargyline: a tool for studying the synthesis of the enzyme.
Mol Pharmacol. 1971 Mar;7(2):219-28.
Zotero 插件