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本文引用的文献

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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
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Choline uptake by the neuroblastoma x glioma hybrid, NG108-15.神经母细胞瘤与胶质瘤杂交细胞NG108-15对胆碱的摄取。
J Neurochem. 1980 Oct;35(4):829-37. doi: 10.1111/j.1471-4159.1980.tb07079.x.
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Choline high-affinity uptake and metabolism and choline acetyltransferase activity in the striatum of rats chronically treated with neuroleptics.
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Morphological alterations and increase of S-100 protein in cultured human glioma cells deprived of serum.血清剥夺培养的人胶质瘤细胞中S-100蛋白的形态学改变及增加
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Choline uptake in glial cell cultures.胶质细胞培养物中的胆碱摄取。
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Interrelationships among the levels of ATP, adenosine and cyclic AMP in incubated slices of guinea-pig cerebral cortex: effects of depolarizing agents, psychotropic drugs and metabolic inhibitors.
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人胶质瘤细胞培养物中胆碱的摄取与释放

Uptake and release of choline in cultures of human glioma cells.

作者信息

Walum E

机构信息

Unit of Neurochemistry and Neurotoxicology, University of Stockholm, Sundbyberg, Sweden.

出版信息

Cell Mol Neurobiol. 1981 Dec;1(4):389-99. doi: 10.1007/BF00716273.

DOI:10.1007/BF00716273
PMID:6765739
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11572855/
Abstract

Human glioma cells (138MG) have a low-affinity uptake system for choline (Km = 20 microM; Vmax = 56 pmol/min/10(6) cells). The uptake is reduced by acetylcholine, hemicholinium-3, HgCl2, and phosphodiesterase inhibitors. Release of [3H]choline from preloaded cultures showed two pools with half-lives of 1.3 and 160 min. Choline release was stimulated by 8-bromo-cAMP or isobutylmethylxanthine. The results suggest that release of choline occurs by a facilitated diffusion transport system and is increased by elevations of intracellular cAMP.

摘要

人胶质瘤细胞（138MG）具有一个对胆碱的低亲和力摄取系统（Km = 20微摩尔；Vmax = 56皮摩尔/分钟/10⁶个细胞）。摄取会被乙酰胆碱、半胱氨酸-3、HgCl₂和磷酸二酯酶抑制剂所降低。从预加载培养物中释放[³H]胆碱显示出两个池，半衰期分别为1.3分钟和160分钟。胆碱释放受到8-溴-cAMP或异丁基甲基黄嘌呤的刺激。结果表明，胆碱的释放是通过易化扩散转运系统发生的，并且细胞内cAMP升高会使其增加。