人胶质瘤细胞培养物中胆碱的摄取与释放

Uptake and release of choline in cultures of human glioma cells.

作者信息

Walum E

机构信息

Unit of Neurochemistry and Neurotoxicology, University of Stockholm, Sundbyberg, Sweden.

出版信息

Cell Mol Neurobiol. 1981 Dec;1(4):389-99. doi: 10.1007/BF00716273.

DOI:10.1007/BF00716273

PMID:6765739

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11572855/

Abstract

Human glioma cells (138MG) have a low-affinity uptake system for choline (Km = 20 microM; Vmax = 56 pmol/min/10(6) cells). The uptake is reduced by acetylcholine, hemicholinium-3, HgCl2, and phosphodiesterase inhibitors. Release of [3H]choline from preloaded cultures showed two pools with half-lives of 1.3 and 160 min. Choline release was stimulated by 8-bromo-cAMP or isobutylmethylxanthine. The results suggest that release of choline occurs by a facilitated diffusion transport system and is increased by elevations of intracellular cAMP.

摘要

人胶质瘤细胞（138MG）具有一个对胆碱的低亲和力摄取系统（Km = 20微摩尔；Vmax = 56皮摩尔/分钟/10⁶个细胞）。摄取会被乙酰胆碱、半胱氨酸-3、HgCl₂和磷酸二酯酶抑制剂所降低。从预加载培养物中释放[³H]胆碱显示出两个池，半衰期分别为1.3分钟和160分钟。胆碱释放受到8-溴-cAMP或异丁基甲基黄嘌呤的刺激。结果表明，胆碱的释放是通过易化扩散转运系统发生的，并且细胞内cAMP升高会使其增加。