脊髓灰质炎病毒的噬斑形成及纯系分离
Plaque formation and isolation of pure lines with poliomyelitis viruses.
作者信息
DULBECCO R, VOGT M
出版信息
J Exp Med. 1954 Feb;99(2):167-82. doi: 10.1084/jem.99.2.167.
Abstract
Plaques have been produced with the three types of poliomyelitis viruses on monolayer tissue cultures of monkey kidney and monkey testis. The number of plaques was proportional to the concentration of the virus. Each plaque originates, therefore, from a single virus particle, defined as the virus unit that is unseparable by dilution. The plaques are due to the specific action of the virus since they are suppressed by type-specific antiserum. Pure virus lines were established by isolating the virus population produced in single plaques. These derived virus lines had the same morphological, serological, and pathogenic properties as the parent strain. High titer virus stocks, with titers up to 7 x 10(8) plaque-forming particles per ml., were obtained.
摘要
已使用三种脊髓灰质炎病毒在猴肾和猴睾丸的单层组织培养物上产生了蚀斑。蚀斑数量与病毒浓度成正比。因此，每个蚀斑都源自单个病毒颗粒，单个病毒颗粒被定义为无法通过稀释分离的病毒单位。蚀斑是由病毒的特异性作用引起的，因为它们会被型特异性抗血清抑制。通过分离单个蚀斑中产生的病毒群体建立了纯病毒株系。这些衍生的病毒株系具有与亲本菌株相同的形态学、血清学和致病性特性。获得了高滴度病毒储备液，滴度高达每毫升7×10⁸个蚀斑形成颗粒。
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