Hurst P R, Wheeler A G, Eckstein P
Fertil Steril. 1980 Jan;33(1):69-76. doi: 10.1016/s0015-0282(16)44482-1.
Fifteen mature female rhesus monkeys were mated and operated upon to recover preimplantation uterine embryos. This resulted in the recovery of 25 embryos or unfertilized ava in 43 attempts. The same animals were fitted with plastic intrauterine devices (IUDs) and the recovery procedure was repeated. In this series 61 recovery attempts yielded only 10 embryos, 7 of which were sectioned and analyzed by transmission electron microscopy. This approach revealed the ultrastructural details of embryo degeneration and leukocyte association seen in embryos recovered from IUD-bearing animals. Samples of peripheral venous blood were analyzed for levels of ovarian steroids at the time of embryo recovery. The results are discussed in relation to the mechanism of action of IUDs.
15只成年雌性恒河猴交配后接受手术以获取植入前的子宫胚胎。在43次尝试中成功获取了25个胚胎或未受精卵。给这些相同的动物植入塑料宫内节育器（IUD），并重复胚胎获取过程。在这一系列操作中，61次获取尝试仅得到10个胚胎，其中7个经切片后用透射电子显微镜进行分析。这种方法揭示了从携带IUD的动物体内获取的胚胎中所观察到的胚胎退化和白细胞关联的超微结构细节。在获取胚胎时，分析外周静脉血样本中卵巢甾体激素的水平。结合IUD的作用机制对结果进行了讨论。
