Kasai R, Nozawa Y
Biochim Biophys Acta. 1980 Jan 18;617(1):161-6. doi: 10.1016/0005-2760(80)90234-9.
The regulatory mechanism of palmitoyl-CoA desaturase activity in the downward temperature shift was investigated in the non-growing (starved) Tetrahymena cells. This cell system is advantageous in allowing depletion of the pre-existing palmitoyl-CoA desaturase. An unexpectedly profound increase in its activity was observed following temperature shift from 34 degrees C to 15 degrees C, providing evidence to support the concept that the increased level of the desaturase activity results from induction of the desaturase synthesis, probably by reutilization of degraded cell components, rather than from direct membrane fluidity control as observed in different experiments.
在非生长（饥饿）的四膜虫细胞中研究了棕榈酰辅酶A去饱和酶活性在温度下降时的调节机制。这个细胞系统有利于耗尽预先存在的棕榈酰辅酶A去饱和酶。在温度从34℃转变为15℃后，观察到其活性意外地大幅增加，这为支持以下概念提供了证据：去饱和酶活性水平的提高是由于去饱和酶合成的诱导，可能是通过降解细胞成分的再利用，而不是如在不同实验中观察到的由直接的膜流动性控制所致。
