Robinson J M, Karnovsky M J
J Histochem Cytochem. 1980 Feb;28(2):161-8. doi: 10.1177/28.2.6766487.
Distribution of the filipin-cholesterol complexes has been studied by freeze-fractures techniques. While filipin-cholesterol complexes are formed in fixed as well as unfixed cells, the distribution of the complexes varies with fixation. Furthermore, filipin induces dramatic rearrangement of intramembrane particles in unfixed, but not in fixed, cells. Examination of thin sections of filipin-treated cells shows that filipin alone induces substantial cellular damage, while filipin treatment simultaneous to fixation or following fixation does not cause noticeable damage. For filipin to be an effective cytochemical probe of membrane structure, cellular damage must be minimized; this is best achieved by treating cells with filipin following glutaraldehyde fixation.
已通过冷冻断裂技术研究了制霉菌素-胆固醇复合物的分布。虽然制霉菌素-胆固醇复合物在固定和未固定的细胞中均会形成，但复合物的分布会因固定方式而异。此外，制霉菌素会诱导未固定细胞而非固定细胞中的膜内颗粒发生显著重排。对经制霉菌素处理的细胞薄片进行检查表明，单独使用制霉菌素会导致大量细胞损伤，而在固定同时或固定后进行制霉菌素处理则不会造成明显损伤。为使制霉菌素成为有效的膜结构细胞化学探针，必须将细胞损伤降至最低；最好的方法是在戊二醛固定后用制霉菌素处理细胞。
