Ferszt R, Neu S, Cervós-Navarro J, Sperner J
Acta Neuropathol. 1978 Jun 30;42(3):223-9. doi: 10.1007/BF00690361.
Focal brain edema limited to one cerebral hemisphere was produced by ultraviolet irradiation of the exposed cortex. Tissue water content was determined by the gravimetric method which allows microsampling. Therefore, the spread of edema around the small necrotic area could be mapped more precisely than by determination of dry weight which calls for larger samples. As early as 30 min following irradiation, hyperemia and swelling of the brain are observed under the operating microscope. This correlates with venous stasis, hyperemia, and broadened perivascular spaces around venules and large capillaries accompanied by a marked rise in the specific weight of the tissue. After 4 h an edema front can be observed spreading from the perinecrotic zone in which there is a marked rise in endothelial cell vesicular activity. Edema reaches maximum levels in the deep white matter at 48 h post irradiation with normalisation of the tissue water content after 96 h. The velocity at which the edema front spreads from the cortex to the periventricular area lies in the range of 0.25 mm/hr. Edema reabsorption coincides with signs of retrograde micropinocytosis in endothelial cells.
通过对暴露的皮质进行紫外线照射，在一个脑半球产生局限性脑水肿。组织含水量采用重量法测定，该方法允许进行微量采样。因此，与需要更大样本的干重测定相比，能够更精确地绘制小坏死区域周围水肿的扩散情况。照射后30分钟，在手术显微镜下即可观察到脑充血和肿胀。这与静脉淤滞、充血以及小静脉和大毛细血管周围血管周围间隙增宽相关，同时组织比重显著升高。4小时后，可观察到水肿前沿从坏死周边区域扩散，该区域内皮细胞泡状活动显著增强。照射后48小时，水肿在深部白质达到最大程度，96小时后组织含水量恢复正常。水肿前沿从皮质扩散到脑室周围区域的速度在0.25毫米/小时范围内。水肿吸收与内皮细胞逆行微胞饮作用的迹象一致。
