转化DNA中化学诱变对部分二倍体供体的需求。
Requirement of a partially diploid donor for the chemical induction of mutations in transforming DNA.
作者信息
Heard J T, Matney T S
出版信息
Mutat Res. 1980 Feb;69(2):217-24. doi: 10.1016/0027-5107(80)90086-x.
PMID:6767181
Abstract
In the Bacillus subtilis transformation system, in vitro chemical mutagenesis may depend upon the use of a DNA donor carrying two copies of the region of interest. Transformations involving this region of the donor DNA which has been chemically treated may lead to the recovery of new mutations or segregant products by some as yet undetermined mechanism.
摘要
在枯草芽孢杆菌转化系统中，体外化学诱变可能依赖于使用携带感兴趣区域两个拷贝的DNA供体。涉及经过化学处理的供体DNA该区域的转化可能通过某种尚未确定的机制导致新突变或分离产物的获得。
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