Koivusaari U, Lang M, Hietanen E
Acta Pharmacol Toxicol (Copenh). 1980 Jan;46(1):37-42. doi: 10.1111/j.1600-0773.1980.tb02416.x.
The activities of the microsomal drug metabolizing enzymes in the liver and intestinal mucosa of rats were studied after the intraperitoneal administration of carbon tetrachloride and/or subcutaneous phenobarbital administration. The membrane phospholipid content was decreased after carbon tetrachloride treatment indicating destruction in the membrane structure. Aryl hydrocarbon hydroxylase activity was decreased in the liver and intestinal mucosa after treatment with CCl4 alone and in combination with phenobarbital. The CCl4 treatment increased the intestinal epoxide hydratase activity but decreased the activity in the liver. The hepatic UDPglucuronosyltransferase was slightly induced by phenobarbital and the activity was elevated by the CCl4 treatment. In the intestinal mucosa the enhanced UDPglucuronosyltransferase activity was observed only after phenobarbital pretreatment and the activity was decreased by CCl4. These results support the view that epoxide hydratase and UDPglucuronosyltransferase enzymes occupy different locations in the endosplasmic reticulum of intestinal mucosa than of liver.
在大鼠腹腔注射四氯化碳和/或皮下注射苯巴比妥后，研究了其肝脏和肠黏膜中微粒体药物代谢酶的活性。四氯化碳处理后膜磷脂含量降低，表明膜结构遭到破坏。单独用四氯化碳处理以及四氯化碳与苯巴比妥联合处理后，肝脏和肠黏膜中的芳烃羟化酶活性均降低。四氯化碳处理增加了肠道环氧化物水化酶的活性，但降低了肝脏中的该酶活性。苯巴比妥可轻微诱导肝脏中的尿苷二磷酸葡萄糖醛酸基转移酶，四氯化碳处理可提高其活性。在肠黏膜中，仅在苯巴比妥预处理后观察到尿苷二磷酸葡萄糖醛酸基转移酶活性增强，而四氯化碳可降低该活性。这些结果支持以下观点：环氧化物水化酶和尿苷二磷酸葡萄糖醛酸基转移酶在肠黏膜内质网中的定位与在肝脏内质网中的不同。
