Thau R B, Sundaram K
Fertil Steril. 1980 Mar;33(3):317-20. doi: 10.1016/s0015-0282(16)44601-7.
Active immunization of female rhesus monkeys with the beta-subunit of ovine luteinizing hormone )oLH beta) significantly reduced their fertility. To determine whether the major action of the vaccine was interruption of pregnancy, by suppression of "corpus luteum rescue," or inhibition of ovulation, we administered the progestational agent medroxyprogesterone acetate (MPA) from day 4 through day 40 after mating. In the untreated immunized group, the pregnancy rate was significantly below that of control monkeys. MPA treatment restored the fertility rate of immunized animals to that of the control group. These results strongly support the assumption that the antifertility action of antibodies of oLH beta is due to prevention of corpus luteum rescue. Whether the lack of corpus luteum rescue resulted because of neutralization of rhesus monkey chorionic gonadotropin or from a defective corpus luteum, as is found in animals with short luteal phases, cannot be determined from these studies. The successful reversal of the antifertility effect, however, suggests that the circulating antibodies do not interfere with normal ovulation or with the normal development and implantation of the blastocyst.
用绵羊促黄体生成素β亚基（oLHβ）对雌性恒河猴进行主动免疫可显著降低其生育能力。为了确定该疫苗的主要作用是通过抑制“黄体拯救”来中断妊娠，还是抑制排卵，我们在交配后第4天至第40天给予孕激素醋酸甲羟孕酮（MPA）。在未治疗的免疫组中，妊娠率显著低于对照猴。MPA治疗使免疫动物的生育率恢复到对照组水平。这些结果有力地支持了这样一种假设，即oLHβ抗体的抗生育作用是由于阻止了黄体拯救。从这些研究中无法确定黄体拯救的缺乏是由于恒河猴绒毛膜促性腺激素的中和作用，还是如在黄体期短的动物中发现的那样，是由于黄体缺陷所致。然而，抗生育作用的成功逆转表明，循环抗体不会干扰正常排卵或胚泡的正常发育和着床。
