Brawer J R, Ruf K B, Naftolin F
Neuroendocrinology. 1980;30(3):144-9. doi: 10.1159/000122990.
Female Wistar rats treated with a single subcutaneous injection of 2 mg estradiol valerate (EV) develop gradually progressive, multifocal lesions of the arcuate nucleus. They also exhibit vaginal estrus and endocrine profiles characteristic of animals sustaining anterior hypothalamic deafferentation. In this study, EV-treated females with the arcuate lesions released significantly less LH 1 h following electrochemical stimulation of the medial preoptic area (MPOA) than did normally cycling controls in proestrus. FSH release in response to MPOA stimulation was the same for both groups. As plasma LH concentrations were not significantly different between EV-treated and control animals 1 h after the injection of a potent LHRH analog, the reduced LH response to MPOA stimulation appears to reflect a primarily hypothalamic defect. However, the EV treatment also affected pituitary responsiveness to long-term stimulation as evidenced by reduced LH responses to the LHRH analog after 2 and 3 h. No such differences were seen in the FSH response.
经皮下单次注射2mg戊酸雌二醇（EV）处理的雌性Wistar大鼠会逐渐出现弓状核的进行性多灶性病变。它们还表现出阴道发情以及维持下丘脑前部去传入动物所特有的内分泌特征。在本研究中，与处于发情前期的正常周期对照相比，经EV处理且有弓状核病变的雌性大鼠在对内侧视前区（MPOA）进行电化学刺激1小时后释放的促黄体生成素（LH）显著减少。两组对MPOA刺激的促卵泡生成素（FSH）释放情况相同。由于在注射强效促性腺激素释放激素（LHRH）类似物1小时后，经EV处理的动物与对照动物的血浆LH浓度无显著差异，因此LH对MPOA刺激反应的降低似乎主要反映了下丘脑缺陷。然而，EV处理也影响了垂体对长期刺激的反应能力，这在2小时和3小时后对LHRH类似物的LH反应降低中得到了证实。在FSH反应中未观察到此类差异。
