枯草芽孢杆菌中核糖体蛋白的甲基化作用

Methylation of ribosomal proteins in Bacillus subtilis.

作者信息

Mardones E, Amaro A M, Jerez C A

出版信息

J Bacteriol. 1980 Apr;142(1):355-8. doi: 10.1128/jb.142.1.355-358.1980.

DOI:10.1128/jb.142.1.355-358.1980

PMID:6768716

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC293973/

Abstract

We measured the methylation of ribosomal proteins from the 30S and 50S subunits of Bacillus subtilis after growing the cells in the presence of [1-14C]methionine and [methyl-3H]methionine. Two-dimensional polyacrylamide gel electrophoretic analysis revealed a preferential methylation of the 50S ribosomal proteins. Proteins L11 and L16, and possibly L9, L10, L18, and L20, were methylated. On the other hand, only two possibly methylated proteins were found on the 30S subunit. A comparison of these results with those for Escherichia coli suggests a common methylation pattern for the bacterial ribosomal proteins.

摘要

在含有[1-¹⁴C]甲硫氨酸和[甲基-³H]甲硫氨酸的条件下培养枯草芽孢杆菌细胞后，我们测定了来自其30S和50S亚基的核糖体蛋白的甲基化情况。二维聚丙烯酰胺凝胶电泳分析显示50S核糖体蛋白存在优先甲基化现象。蛋白L11和L16，可能还有L9、L10、L18和L20发生了甲基化。另一方面，在30S亚基上仅发现了两种可能发生甲基化的蛋白。将这些结果与大肠杆菌的结果进行比较，提示细菌核糖体蛋白存在共同的甲基化模式。