大肠杆菌中核糖体蛋白的甲基化作用。
Methylation of ribosomal proteins in Escherichia coli.
作者信息
Chang F N, Chang C N, Paik W K
出版信息
J Bacteriol. 1974 Nov;120(2):651-6. doi: 10.1128/jb.120.2.651-656.1974.
Abstract
Escherichia coli was grown in a medium containing [1-(14)C]methionine and [methyl-(3)H]methionine, and the (3)H/(14)C ratio was determined for each of the ribosomal proteins derived from the 70S ribosome. Evidence indicates that six proteins from the 50S subunit were methylated: L7, L9, L11, L12, L18, and L33. Methylation of several other 50S proteins (such as L1, L3, L5, etc.) may also occur. The methylated amino acids in protein L11 have been characterized further and found to be predominately epsilon-trimethyllysine. A small amount of a compound tentatively identified as N(G), N'(G)-dimethylarginine was also detected.
摘要
将大肠杆菌培养在含有[1-(14)C]甲硫氨酸和[甲基-(3)H]甲硫氨酸的培养基中，并测定了源自70S核糖体的每种核糖体蛋白的(3)H/(14)C比值。有证据表明，50S亚基的六种蛋白质发生了甲基化：L7、L9、L11、L12、L18和L33。其他几种50S蛋白质（如L1、L3、L5等）也可能发生甲基化。蛋白质L11中的甲基化氨基酸已得到进一步鉴定，发现主要是ε-三甲基赖氨酸。还检测到少量初步鉴定为N(G)，N'(G)-二甲基精氨酸的化合物。
相似文献
1
Methylation of ribosomal proteins in Escherichia coli.大肠杆菌中核糖体蛋白的甲基化作用。
J Bacteriol. 1974 Nov;120(2):651-6. doi: 10.1128/jb.120.2.651-656.1974.
2
Methylation of the ribosomal proteins in Escherichia coli. Nature and stoichiometry of the methylated amino acids in 50S ribosomal proteins.大肠杆菌中核糖体蛋白的甲基化。50S核糖体蛋白中甲基化氨基酸的性质和化学计量。
Biochemistry. 1975 Feb 11;14(3):468-77. doi: 10.1021/bi00674a002.
3
Genetics of ribosomal protein methylation in Escherichia coli. I. A mutant deficient in methylation of protein L11.
Mol Gen Genet. 1977 Jul 20;154(2):167-73. doi: 10.1007/BF00330832.
4
Methylation of ribosomal proteins during ribosome assembly in Escherichia coli.大肠杆菌核糖体组装过程中核糖体蛋白的甲基化作用。
Mol Gen Genet. 1981;183(3):418-21. doi: 10.1007/BF00268759.
5
The requirement for ribosomal proteins L7 and L12 in peptide-chain termination.肽链终止过程中对核糖体蛋白L7和L12的需求。
Proc Natl Acad Sci U S A. 1974 Jan;71(1):89-92. doi: 10.1073/pnas.71.1.89.
6
Characterization of methylated neutral amino acids from Escherichia coli ribosomes.大肠杆菌核糖体中甲基化中性氨基酸的特性分析。
J Bacteriol. 1977 Jul;131(1):105-10. doi: 10.1128/jb.131.1.105-110.1977.
7
A comparative study of the 50S ribosomal subunit and several 50S subparticles in EF-T-and EF-G-dependent activities.50S核糖体亚基及若干50S亚颗粒在依赖EF-T和EF-G的活性方面的比较研究
Biochemistry. 1975 May 6;14(9):1805-14. doi: 10.1021/bi00680a001.
8
Ribosomal protein L7/L12 cross-links to proteins in separate regions of the 50 S ribosomal subunit of Escherichia coli.核糖体蛋白L7/L12与大肠杆菌50 S核糖体亚基不同区域的蛋白质发生交联。
J Biol Chem. 1983 Dec 10;258(23):14592-8.
9
Exchange of ribosomal proteins among the ribosomes of Escherichia coli.
Mol Gen Genet. 1977 Nov 29;157(2):205-14. doi: 10.1007/BF00267399.
10
Assembly of ribosomal proteins L7, L10, L11, and L12, on the 50 S subunit of Escherichia coli.
J Biol Chem. 1975 Feb 10;250(3):813-4.
引用本文的文献
1
aKMT Catalyzes Extensive Protein Lysine Methylation in the Hyperthermophilic Archaeon Sulfolobus islandicus but is Dispensable for the Growth of the Organism.aKMT催化嗜热古菌冰岛硫化叶菌中的广泛蛋白质赖氨酸甲基化，但对该生物体的生长并非必需。
Mol Cell Proteomics. 2016 Sep;15(9):2908-23. doi: 10.1074/mcp.M115.057778. Epub 2016 Jun 21.
2
Pseudomonas aeruginosa EftM Is a Thermoregulated Methyltransferase.铜绿假单胞菌EftM是一种温度调节型甲基转移酶。
J Biol Chem. 2016 Feb 12;291(7):3280-90. doi: 10.1074/jbc.M115.706853. Epub 2015 Dec 16.
3
Identification and characterization of a highly conserved crenarchaeal protein lysine methyltransferase with broad substrate specificity.鉴定并阐明具有广泛底物特异性的高度保守的古菌蛋白赖氨酸甲基转移酶。
J Bacteriol. 2012 Dec;194(24):6917-26. doi: 10.1128/JB.01535-12. Epub 2012 Oct 19.
4
Methylation of ribosomal proteins during ribosome assembly in Escherichia coli.大肠杆菌核糖体组装过程中核糖体蛋白的甲基化作用。
Mol Gen Genet. 1981;183(3):418-21. doi: 10.1007/BF00268759.
5
Methylation of ribosomal proteins in Bacillus subtilis.枯草芽孢杆菌中核糖体蛋白的甲基化作用
J Bacteriol. 1980 Apr;142(1):355-8. doi: 10.1128/jb.142.1.355-358.1980.
6
Correlation between RNA synthesis and ppGpp content in Escherichia coli during temperature shifts.温度变化期间大肠杆菌中RNA合成与ppGpp含量之间的相关性
Mol Gen Genet. 1983;192(1-2):5-9. doi: 10.1007/BF00327639.
7
Influence of amino acid starvation on guanosine 5'-diphosphate 3'-diphosphate basal-level synthesis in Escherichia coli.氨基酸饥饿对大肠杆菌中鸟苷5'-二磷酸3'-二磷酸基础水平合成的影响。
J Bacteriol. 1980 Nov;144(2):499-508. doi: 10.1128/jb.144.2.499-508.1980.
8
The role of guanosine-3',5'-bis-pyrophosphate in mediating antimicrobial activity of the antibiotic 3,5-dihydroxy-4-ethyl-trans-stilbene.鸟苷-3',5'-二磷酸在介导抗生素3,5-二羟基-4-乙基反式芪抗菌活性中的作用
Antimicrob Agents Chemother. 1992 Dec;36(12):2645-51. doi: 10.1128/AAC.36.12.2645.
9
Modification of yeast ribosomal proteins. Methylation.酵母核糖体蛋白的修饰。甲基化。
Biochem J. 1978 Oct 1;175(1):221-5. doi: 10.1042/bj1750221.
10
Modification of yeast ribosomal proteins. Phosphorylation.酵母核糖体蛋白的修饰。磷酸化。
Biochem J. 1978 Oct 1;175(1):213-9. doi: 10.1042/bj1750213.
本文引用的文献
1
Bacterial ribosome.细菌核糖体
Bacteriol Rev. 1970 Sep;34(3):228-77. doi: 10.1128/br.34.3.228-277.1970.
2
The dependence of cell-free protein synthesis in E. coli upon naturally occurring or synthetic polyribonucleotides.大肠杆菌中无细胞蛋白质合成对天然存在或合成的多聚核糖核苷酸的依赖性。
Proc Natl Acad Sci U S A. 1961 Oct 15;47(10):1588-602. doi: 10.1073/pnas.47.10.1588.
3
STREPTOMYCIN ACTION AND THE RIBOSOME.链霉素的作用与核糖体
Proc Natl Acad Sci U S A. 1964 Apr;51(4):703-9. doi: 10.1073/pnas.51.4.703.
4
Omega-N-methylarginine in protein.
J Biol Chem. 1970 Jan 10;245(1):88-92.
5
The methylation of lysine residues in protein.蛋白质中赖氨酸残基的甲基化。
J Biol Chem. 1966 Apr 25;241(8):1857-62.
6
Protein methylation.蛋白质甲基化
Science. 1971 Oct 8;174(4005):114-9. doi: 10.1126/science.174.4005.114.
7
Structure and function of bacterial ribosomes. XII. Accumulation of 21 s particles by some cold-sensitive mutants of Escherichia coli.细菌核糖体的结构与功能。十二。大肠杆菌某些冷敏感突变体对21 s颗粒的积累。
J Mol Biol. 1971 Nov 28;62(1):121-38. doi: 10.1016/0022-2836(71)90135-5.
8
Purification and characterization of 50S ribosomal proteins of Escherichia coli.大肠杆菌50S核糖体蛋白的纯化与特性分析
Mol Gen Genet. 1971;112(3):229-42. doi: 10.1007/BF00269176.
9
Ribosomal proteins. XV. Amino acid compositions of isolated ribosomal proteins from 30S and 50S subunits of Escherichia coli.核糖体蛋白。十五。大肠杆菌30S和50S亚基中分离出的核糖体蛋白的氨基酸组成。
Mol Gen Genet. 1970;109(4):292-7. doi: 10.1007/BF00267698.
10
Ribosomal proteins. 8. Molecular weights of isolated ribosomal proteins of Escherichia coli.核糖体蛋白。8. 大肠杆菌分离核糖体蛋白的分子量。
Proc Natl Acad Sci U S A. 1970 Dec;67(4):1909-13. doi: 10.1073/pnas.67.4.1909.
Zotero 插件