Zhuravskiĭ N I, Komissarenko S V, Mel'nichuk D A, Lukinov D I, MOrgunov I N
Ukr Biokhim Zh (1978). 1980 Jan-Feb;52(1):103-9.
The paper deals with dynamics of antibody formation in rats. Tetanic anatoxin independently of doses and methods of administration manifests weak immunogenic properties. A complete Freind adjuvant stimulates considerably the biosynthesis of antitetanic antibodies in the primary and secondary immune responses. Electrophoresis of tetanic anatoxin polyacrylamide gel permits isolating two protein fractions, one of them migrates in the immunoglobulin G zone and its mass constitutes 73%, the other-more low-molecular and contains 27% of protein. Dynamics of the acid-base blood state indices and of antibody biosynthesis is studied on models of chronic compensated alkalosis and metabolic acidosis in rats. It is established that during the experiment, simultaneously with an insignificant increase in the total carbonic acid concentration blood, there occurs a 25-60% increase in the titres of antitetanic antibodies as compared to the control. In rats with acidosis there occurs a simultaneously decrease in the intensity of antibody biosynthesis as compared to the control (43-54%) and in the total CO2 content in blood.
本文探讨大鼠体内抗体形成的动力学。破伤风类毒素无论剂量和给药方法如何，其免疫原性都较弱。完全弗氏佐剂在初次和二次免疫反应中能显著刺激抗破伤风抗体的生物合成。破伤风类毒素在聚丙烯酰胺凝胶上进行电泳可分离出两个蛋白质组分，其中一个在免疫球蛋白G区迁移，其质量占73%，另一个分子量较低，含27%的蛋白质。在大鼠慢性代偿性碱中毒和代谢性酸中毒模型上研究了酸碱血液状态指标和抗体生物合成的动力学。结果表明，在实验过程中，与血液中总碳酸浓度的轻微增加同时，抗破伤风抗体滴度比对照组增加了25% - 60%。与对照组相比，酸中毒大鼠体内抗体生物合成强度同时降低（43% - 54%），且血液中总二氧化碳含量也降低。
