Khan M A, Angus B M
Acta Anat (Basel). 1980;106(3):327-9. doi: 10.1159/000145196.
Acid phosphatase activity was studied histochemically in rat livers after perfusion fixation. The three different perfusates used were (1) 2% of paraformaldehyde; (2) 1.25% of glutaraldehyde, and (3) 1.25% of each of paraformaldehyde and glutaraldehyde. Unfixed fresh frozen sections showed high enzymatic activity in both centrilobular (CL) and periportal (PP) areas. A similar localization pattern resulted after perfusate 1 indicating a lack of reduction in the enzymatic activity in either of the two areas. After perfusate 2, PP arease remained intensely stained while slight reduction of the enzymatic activity was exhibited by the CL regions. Perfusion (3) resulted in higher activity in PP areas but maximal inhibition in the CL region. The inhibition of acid phosphatase activity in the CL areas correlates with regional differences of Kupffer cell population as well as the existence of metabolic heterogeneity between the two hepatic regions.
采用组织化学方法研究了灌注固定后大鼠肝脏中的酸性磷酸酶活性。使用的三种不同灌注液分别为：（1）2%多聚甲醛；（2）1.25%戊二醛；（3）2%多聚甲醛与1.25%戊二醛混合液。未固定的新鲜冰冻切片在中央小叶（CL）和汇管区（PP）均显示出高酶活性。灌注液1灌注后呈现出相似的定位模式，表明这两个区域的酶活性均未降低。灌注液2灌注后，PP区仍强烈染色，而CL区的酶活性略有降低。灌注液3灌注后，PP区活性较高，但CL区受到最大抑制。CL区酸性磷酸酶活性的抑制与枯否细胞群体的区域差异以及两个肝区之间代谢异质性的存在相关。
