Boyan-Salyers B D, Boskey A L
Calcif Tissue Int. 1980;30(2):167-74. doi: 10.1007/BF02408622.
Calcium-phospholipid-phosphate complexes (Ca-PL-P) were isolated from calcified and uncalcified Bacterionema matruchotii and its calcified lipid extracts. Similar complexes were absent from the noncalcifying bacterium Actinomyces naeslundii. The majority of the Ca-PL-P complexes were associated with the proteolipid acidic phospholipid component. Ca-PL-P complexes isolated from B. matruchotii and from calcified proteolipid contained phosphatidylinositol, phosphatidylinositol-4-phosphate, phosphatidylinositol-4,5-diphosphate, and phosphatidylserine. They consisted of approximately 52 mole % Ca, 32 mole % organic P, and 15 mole % Pi. During Ca-PL-P extraction from B. matruchotii or its proteolipid-containing calcified lipid extracts, the proteolipid was dissociated and the apoprotein precipitated as fluff at the aqueous-organic solvent interface, thus explaining the failure to detect protein in Ca-PL-P preparations. When the ability of Ca-PL-P complexes and lipid fractions of B. matruchotii to initiate apatite formation from metastable calcium phosphate solution was compared, the yield of hydroxyapatite decreased as follows: Ca-PL-P greater than proteolipid acidic phospholipids greater than proteolipid greater than crude phospholipid greater than total lipids greater than whole cells.
从钙化和未钙化的龋齿杆菌及其钙化脂质提取物中分离出钙 - 磷脂 - 磷酸盐复合物（Ca - PL - P）。非钙化细菌内氏放线菌中不存在类似的复合物。大多数Ca - PL - P复合物与蛋白脂质酸性磷脂成分相关。从龋齿杆菌和钙化蛋白脂质中分离出的Ca - PL - P复合物含有磷脂酰肌醇、磷脂酰肌醇 - 4 - 磷酸、磷脂酰肌醇 - 4,5 - 二磷酸和磷脂酰丝氨酸。它们约由52摩尔%的钙、32摩尔%的有机磷和15摩尔%的无机磷组成。在从龋齿杆菌或其含蛋白脂质的钙化脂质提取物中提取Ca - PL - P的过程中，蛋白脂质解离，载脂蛋白在水 - 有机溶剂界面沉淀成绒毛状，这就解释了在Ca - PL - P制剂中未能检测到蛋白质的原因。当比较龋齿杆菌的Ca - PL - P复合物和脂质部分从亚稳态磷酸钙溶液引发磷灰石形成的能力时，羟基磷灰石的产量如下降低：Ca - PL - P＞蛋白脂质酸性磷脂＞蛋白脂质＞粗磷脂＞总脂质＞全细胞。
