Nur I, Okon Y, Henis Y
Can J Microbiol. 1980 Apr;26(4):482-5. doi: 10.1139/m80-080.
Bacteria belonging to the genus Azospirillum isolated from Cynodon dactylon roots in Israel were compared with Azospirillum brasilense from Brazil and California for their ability to fix nitrogen in association with grasses under greenhouse conditions. The plants were grown in a system which avoided cross inoculation from the inoculated soil to the control, while maintaining the natural soil microflora and humidity level in the soil close to field capacity. The organisms tested significantly increased the dry weight of Zea mays and Setaria italica leaves, the total nitrogen content of these leaves (as measured by the Kjeldahl method), and supported acetylene reduction in intact nonsterile systems as compared with the noninoculated controls. Ethylene production in intact systems could be detected after 6h and was linear for 72h, providing a constant soil temperature (28-32 degrees C) was maintained.
从以色列狗牙根根部分离出的固氮螺菌属细菌，与来自巴西和加利福尼亚的巴西固氮螺菌相比较，研究它们在温室条件下与禾本科植物共生固氮的能力。植物种植在一个避免接种土壤与对照之间交叉接种的系统中，同时保持土壤中的自然土壤微生物区系和湿度水平接近田间持水量。与未接种的对照相比，所测试的微生物显著增加了玉米和粟叶片的干重、这些叶片的总氮含量（通过凯氏定氮法测定），并支持完整非无菌系统中的乙炔还原。在完整系统中，6小时后可检测到乙烯产生，在保持恒定土壤温度（28 - 32摄氏度）的情况下，72小时内呈线性，。
