Griffin-Shea R, Thireos G, Kafatos F C, Petri W H, Villa-Komaroff L
Cell. 1980 Apr;19(4):915-22. doi: 10.1016/0092-8674(80)90083-5.
Clones corresponding to two distinct A1 and A2 chorion genes have been isolated from a cDNA library in Drosophila melanogaster and characterized by hybrid-selected translation and blotting-hybridization analysis. These sequences detectably cross hybridize, thus indicating that at least some chorion genes in the fruit fly are homologous. According to in situ hybridization results, the A1 and A2 genes are not linked (mapping in regions 66D 10-12 and 54C-D of the third and second chromosomes, respectively). In conjunction with other evidence, these results suggest that in Drosophila, clustering of chorion genes may be limited to genes which are expressed in parallel during development.
已从黑腹果蝇的一个cDNA文库中分离出与两个不同的A1和A2绒毛膜基因相对应的克隆，并通过杂交选择翻译和印迹杂交分析对其进行了表征。这些序列可检测到交叉杂交，因此表明果蝇中至少一些绒毛膜基因是同源的。根据原位杂交结果，A1和A2基因不连锁（分别定位于第三和第二染色体的66D 10-12和54C-D区域）。结合其他证据，这些结果表明，在果蝇中，绒毛膜基因的聚类可能仅限于在发育过程中平行表达的基因。
