Hiramori K, Sumiyoshi T, Motegi S, Honda T, Kimata S, Hirosawa K, Kawai H, Kondo A, Iwaasa M, Miyoshi K
Am Heart J. 1978 Aug;96(2):187-90. doi: 10.1016/0002-8703(78)90084-4.
A counterimmunoelectrophoresis technique for detection of serum myoglobin (Mb) was improved using non-ionic polymer dextran. Precipitin lines were graded according to their strength, which was ascertained by radioimmunoassay data. By this method, serum Mb in concentrations of 500 ng./ml. before stain and of 200 ng./ml. after stain were detected. Electrophoretic time was 60 minutes. Among 32 cases of acute myocardial infarction (AMI) whose blood samples were collected within 24 hours after disease onset, precipitin lines were detected in 25 cases (78%) before stain and 31 cases (97%) after stain. Considering the early peak concentration time (approximately 10 hours) of serum Mb after AMI onset, diagnosis becomes more rapid and exact with this method, especially in severe cases.
采用非离子聚合物葡聚糖改进了检测血清肌红蛋白（Mb）的对流免疫电泳技术。沉淀线根据其强度分级，强度由放射免疫测定数据确定。用这种方法，检测到染色前血清Mb浓度为500 ng/ml，染色后为200 ng/ml。电泳时间为60分钟。在发病后24小时内采集血样的32例急性心肌梗死（AMI）患者中，染色前25例（78%）检测到沉淀线，染色后31例（97%）检测到沉淀线。考虑到AMI发病后血清Mb的早期峰值浓度时间（约10小时），用这种方法诊断更快速、准确，尤其是在重症病例中。
