Stearne L E, Birkbeck T H
J Med Microbiol. 1980 May;13(2):223-30. doi: 10.1099/00222615-13-2-223.
Native staphylococcal delta haemolysin was poorly immunogenic in the mouse. After treatment with formaldehyde at pH 5 or pH 7.5, haemolytic activity was rapidly lost but antigenic reactivity retained and, at pH 5, immunogenicity was enhanced. Treatment of the haemolysin with formaldehyde at pH 9.5 reduced haemolytic activity by 97% but did not enhance immunogenicity. There was no evidence of polymerisation of formaldehyde-treated delta haemolysin, and the enhanced immunogenicity was considered to be partly due to a reduced affinity for phospholipids.
天然葡萄球菌δ溶血素在小鼠体内免疫原性较差。在pH 5或pH 7.5条件下用甲醛处理后，溶血活性迅速丧失，但抗原反应性得以保留，且在pH 5时免疫原性增强。在pH 9.5条件下用甲醛处理溶血素，溶血活性降低了97%，但未增强免疫原性。没有证据表明甲醛处理过的δ溶血素会发生聚合，增强的免疫原性被认为部分是由于对磷脂的亲和力降低所致。
