Tiunova N A, Rodionova N A, Martinovich L I, Gogolev M N
Prikl Biokhim Mikrobiol. 1980 Mar-Apr;16(2):185-90.
The ability of different fungi cultivated on cellulose to synthesize extracellular cellulase was examined. The fungus Geotrichum candidum str. 3c was the most active among the fungi tested, including Trichoderma fungi. The formation of cellulolytic enzymes in the culture filtrate of G. candidum occurred, when the entire glucose and the major portion of reducing substances were utilized. The formation of cellulases depended on the content of ammonium ions. The enzyme accumulation reached the highest level at the stationary phase of the culture growth and depended on the inoculum amount. When the inoculum was used as a submerged culture at a concentration of 3%, accumulation of C1-enzyme, endo-(1 leads to 4)beta-glucanases, and beta-glucosidases reached maximum by 44, 52 and 56 hours of cultivation. The fats that are frequently used as a foam catcher inhibited the cellulase formation by the abouve strain. From the filtrate cellulolytic enzymes were isolated via spray drying and precipitation with 3 ethanol volumes to yield 6.5 and 5.0 g/l, respectively. The cellulases when stored at 5 degrees C did not lose their activity for a year and became inactivated by 40-50% in 5 years.
研究了在纤维素上培养的不同真菌合成胞外纤维素酶的能力。在包括木霉属真菌在内的受试真菌中，白地霉3c菌株最为活跃。当全部葡萄糖和大部分还原物质被利用时，白地霉培养滤液中会形成纤维素分解酶。纤维素酶的形成取决于铵离子的含量。酶的积累在培养生长的稳定期达到最高水平，并取决于接种量。当接种物以3%的浓度用作深层培养时，在培养44、52和56小时时，C1酶、内切（1→4）β-葡聚糖酶和β-葡萄糖苷酶的积累达到最大值。常用作泡沫捕集器的油脂会抑制上述菌株的纤维素酶形成。通过喷雾干燥和用3倍体积的乙醇沉淀从滤液中分离出纤维素分解酶，产量分别为6.5克/升和5.0克/升。纤维素酶在5℃下储存一年不会失去活性，5年后会失活40 - 50%。
